Construction of a synthetic Saccharomyces cerevisiae pan-genome neo-chromosome

Dariusz R. Kutyna; Cristobal A. Onetto; Thomas C. Williams; Hugh D. Goold; Ian T. Paulsen; Isak S. Pretorius; Daniel L. Johnson; Anthony R. Borneman

doi:10.1038/s41467-022-31305-4

Nature Communications (Jun 2022)

Construction of a synthetic Saccharomyces cerevisiae pan-genome neo-chromosome

Dariusz R. Kutyna,
Cristobal A. Onetto,
Thomas C. Williams,
Hugh D. Goold,
Ian T. Paulsen,
Isak S. Pretorius,
Daniel L. Johnson,
Anthony R. Borneman

Affiliations

Dariusz R. Kutyna: The Australian Wine Research Institute
Cristobal A. Onetto: The Australian Wine Research Institute
Thomas C. Williams: ARC Centre of Excellence in Synthetic Biology and Department of Molecular Sciences, Macquarie University
Hugh D. Goold: ARC Centre of Excellence in Synthetic Biology and Department of Molecular Sciences, Macquarie University
Ian T. Paulsen: ARC Centre of Excellence in Synthetic Biology and Department of Molecular Sciences, Macquarie University
Isak S. Pretorius: ARC Centre of Excellence in Synthetic Biology and Department of Molecular Sciences, Macquarie University
Daniel L. Johnson: The Australian Wine Research Institute
Anthony R. Borneman: The Australian Wine Research Institute

DOI: https://doi.org/10.1038/s41467-022-31305-4
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 9

Abstract

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The Sc2.0 consortia is reengineering the yeast genome. To expand the Sc2.0 genetic repertoire, the authors build a neo-chromosome comprising variable loci from diverse yeast isolates, providing phenotypic plasticity for use in synthetic backgrounds.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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