Nature Communications (Oct 2019)

Monitoring cytosolic H2O2 fluctuations arising from altered plasma membrane gradients or from mitochondrial activity

  • Mercè Carmona,
  • Laura de Cubas,
  • Eric Bautista,
  • Marta Moral-Blanch,
  • Iria Medraño-Fernández,
  • Roberto Sitia,
  • Susanna Boronat,
  • José Ayté,
  • Elena Hidalgo

DOI
https://doi.org/10.1038/s41467-019-12475-0
Journal volume & issue
Vol. 10, no. 1
pp. 1 – 13

Abstract

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Reliable methods of measuring intracellular H2O2 fluctuations are necessary to advance redox biology. Here the authors design a H2O2 sensor based on the fission yeast peroxiredoxin Tpx1 to sense nanomolar fluctuations of intracellular H2O2 in response to genetic and environmental perturbations.