The Impact of BPI Expression on Escherichia coli F18 Infection in Porcine Kidney Cells

Jian Jin; Yanjie Huang; Shouyong Sun; Zhengchang Wu; Shenglong Wu; Zongjun Yin; Wenbin Bao

doi:10.3390/ani10112118

Animals (Nov 2020)

The Impact of BPI Expression on Escherichia coli F18 Infection in Porcine Kidney Cells

Jian Jin,
Yanjie Huang,
Shouyong Sun,
Zhengchang Wu,
Shenglong Wu,
Zongjun Yin,
Wenbin Bao

Affiliations

Jian Jin: Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
Yanjie Huang: Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
Shouyong Sun: Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
Zhengchang Wu: Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
Shenglong Wu: Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
Zongjun Yin: College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China
Wenbin Bao: Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China

DOI: https://doi.org/10.3390/ani10112118
Journal volume & issue: Vol. 10, no. 11
p. 2118

Abstract

Read online

The efficacy and regulatory activity of bactericidal/permeability-increasing protein (BPI) as a mediator of Escherichia coli (E. coli) F18 resistance remains to be defined. In the present study, we evaluated lipopolysaccharide (LPS)-induced changes in BPI gene expression in porcine kidney (PK15) cells in response to E. coli F18 exposure. We additionally generated PK15 cells that overexpressed BPI to assess the impact of this gene on Toll-like receptor 4 (TLR4) signaling and glycosphingolipid biosynthesis-related genes. Through these analyses, we found that BPI expression rose significantly following LPS exposure in response to E. coli F18ac stimulation (p E. coli F18 adherence to PK15 cells was markedly suppressed following BPI overexpression (p BPI overexpression had no significant effect on the mRNA-level expression of genes associated with glycosphingolipid biosynthesis or TLR4 signaling. BPI overexpression suppressed the LPS-induced TLR4 signaling pathway-related expression of proinflammatory cytokines (IFN-α, IFN-β, MIP-1α, MIP-1β and IL-6). Overall, our study serves as an overview of the association between BPI and resistance to E. coli F18 at the cellular level, offering a framework for future investigations of the mechanisms whereby piglets are able to resist E. coli F18 infection.

Published in Animals

ISSN: 2076-2615 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Agriculture: Animal culture: Veterinary medicine; Science: Zoology
Website: http://www.mdpi.com/journal/animals/

About the journal

Abstract

Keywords