In planta transformation of buckwheat (Fagopyrum esculentum Moench.)

Abstract

In order to develop a reliable and rapid transgenic system for functional study of specific buckwheat gene constructs, two different in planta transformation methods were analyzed: vacuum infiltration and infiltration by syringe. The results indicated that the vacuum infiltration method was much more efficient and can therefore be considered the method of choice for buckwheat transformation. .

Keywords

• Buckwheat
• GUS assay
• syringe infiltration
• vacuum infiltration