Mycobiology (Jan 2018)

Development of a Molecular Marker for Fruiting Body Pattern in Auricularia auricula-judae

  • Fang-Jie Yao,
  • Li-Xin Lu,
  • Peng Wang,
  • Ming Fang,
  • You-Min Zhang,
  • Ying Chen,
  • Wei-Tong Zhang,
  • Xiang-Hui Kong,
  • Jia Lu,
  • Yoichi Honda

DOI
https://doi.org/10.1080/12298093.2018.1454004
Journal volume & issue
Vol. 46, no. 1
pp. 72 – 78

Abstract

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The fruiting body pattern is an important agronomic trait of the edible fungus Auricularia auricula-judae, and an important breeding target. There are two types of fruiting body pattern: the cluster type and the chrysanthemum type. We identified the fruiting body pattern of 26 test strains, and then constructed two different near-isogenic pools. Then, we developed sequence characterized amplified region (SCAR) molecular markers associated with the fruiting body pattern based on sequence-related amplified polymorphism (SRAP) markers. Ten different bands (189–522 bp) were amplified using 153 pairs of SRAP primers. The SCAR marker “SCL-18” consisted of a single 522-bp band amplified from the cluster-type strains, but not the chrysanthemum strains. This SCAR marker was closely associated with the cluster-type fruiting body trait of A. auricula-judae. These results lay the foundation for further research to locate and clone genes controlling the fruiting body pattern of A. auricula-judae.

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