Nature Communications (Mar 2019)

Quantifying protein dynamics and stability in a living organism

  • Ruopei Feng,
  • Martin Gruebele,
  • Caitlin M. Davis

DOI
https://doi.org/10.1038/s41467-019-09088-y
Journal volume & issue
Vol. 10, no. 1
pp. 1 – 7

Abstract

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Studying protein kinetics and stability in living organisms is challenging and most studies are performed in cell culture. Here the authors combine meganuclease-mediated transformation and fluorescence-detected temperature-jump microscopy to quantify protein stability in different tissues of living zebrafish.