Data supporting the effects of lysozyme on mRNA and protein expression in a colonic epithelial scratch wound model
Sarah K. Abey,
Yuana Yuana,
Paule V. Joseph,
Natnael D. Kenea,
Nicolaas H. Fourie,
LeeAnne B. Sherwin,
Gregory E. Gonye,
Paul A. Smyser,
Erin S. Stempinski,
Christina M. Boulineaux,
Kristen R. Weaver,
Christopher K.E. Bleck,
Wendy A. Henderson
Affiliations
Sarah K. Abey
Digestive Disorders Unit, Division of Intramural Research, National Institutes of Health, Department of Health and Human Services, Bethesda, MD, USA
Yuana Yuana
Image Sciences Institute, Division of Imaging, University Medical Centre Utrecht, Netherlands
Paule V. Joseph
Digestive Disorders Unit, Division of Intramural Research, National Institutes of Health, Department of Health and Human Services, Bethesda, MD, USA
Natnael D. Kenea
Digestive Disorders Unit, Division of Intramural Research, National Institutes of Health, Department of Health and Human Services, Bethesda, MD, USA
Nicolaas H. Fourie
Digestive Disorders Unit, Division of Intramural Research, National Institutes of Health, Department of Health and Human Services, Bethesda, MD, USA
LeeAnne B. Sherwin
Digestive Disorders Unit, Division of Intramural Research, National Institutes of Health, Department of Health and Human Services, Bethesda, MD, USA
Gregory E. Gonye
NanoString Technologies, Seattle, WA, USA
Paul A. Smyser
The Pennsylvania State University, College of Medicine, Hershey, PA, USA
Erin S. Stempinski
Electron Microscopy Core Facility, National Heart, Lung, and Blood Institute, National Institutes of Health, Department of Health and Human Services, Bethesda, MD, USA
Christina M. Boulineaux
Digestive Disorders Unit, Division of Intramural Research, National Institutes of Health, Department of Health and Human Services, Bethesda, MD, USA
Kristen R. Weaver
Digestive Disorders Unit, Division of Intramural Research, National Institutes of Health, Department of Health and Human Services, Bethesda, MD, USA
Christopher K.E. Bleck
Electron Microscopy Core Facility, National Heart, Lung, and Blood Institute, National Institutes of Health, Department of Health and Human Services, Bethesda, MD, USA
Wendy A. Henderson
Digestive Disorders Unit, Division of Intramural Research, National Institutes of Health, Department of Health and Human Services, Bethesda, MD, USA
Colonic epithelial health is implicated in a host of gastrointestinal (GI) diseases and disorders. Lysozyme is suspected to play a role in the ability of the epithelium to recover from injury (Abey et al., in press; Gallo, 2012; Rubio, 2014) [1–3]. Disrupted repair mechanisms may lead to delayed or ineffective recovery and disruptions to epithelial biology resulting in GI symptoms and altered barrier function (Peterson and Artis, 2014) [4]. The effect of lysozyme on the transcriptomic and proteomic profile of healthy colonic epithelial cells was investigated. Epithelial cells in culture were scratch wounded and treated with lysozyme. mRNA and protein profiles were simultaneously quantified in the same sample using a digital counting technology. Gene and protein expressions altered by the presence or absence of lysozyme are described in this article. Extensive statistical and bioinformatic analysis, and interpretation of the results can be found in “Lysozyme association with circulating RNA, extracellular vesicles, and chronic stress” (Abey et al., in press) [1].
