In vitro potential anthelmintic activity of Biophytum petersianum on Haemonchus contortus

Abstract

Aim: Haemonchus contortus is a major problem in small ruminants in Indonesia. The frequent use of the anthelmintic drugs has given rise to drug-resistant populations which increase the need for new anthelminthic compounds, particularly from endemic plants. This study evaluated the in vitro effects of Biophytum petersianum crude aqueous extract (BAE) as an anthelmintic compound against H. contortus adult worm isolated from goats. Materials and Methods: Adult worm collected from naturally infected abomasums were obtained from slaughtered goats on the day of slaughter. BAE was prepared in six different concentrations (10, 20, 40, 60, 80, and 100 mg/ml) which were tested for their efficacies on ten actively moving worms. Ivermectin (1 mg/ml) was included as a reference drug, while saline water was included as a control. The dead worms from anthelmintic test then went through sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and scanning electron microscopy (SEM). Results: Highest mean mortality in treatments group both at 2 h and 4 h observations was BAE 10%. The SDS-PAGE analysis revealed the presence of five protein bands with molecular weights 9.3, 17.1, 50.0, 63.2, and 72.7 kDa based on BAE 10%. The SEM changes observed in the in vitro trials revealed the occurrence of interactions between the BAE and the cuticle. Conclusion: The SEM and SDS-PAGE analysis revealed ultrastructural structural changes and the decrease numbers of polypeptides on treated worms when compared to the control worms. It can thus be concluded that the BAE exhibits good anthelmintic activity against H. contortus adult worm.

Keywords

• Biophytum petersianum
• crude aqueous extract
• Haemonchus contortus
• scanning electron microscopy
• sodium dodecyl sulfate polyacrylamide gel electrophoresis