High-throughput functional analysis of regulatory variants using a massively parallel reporter assay

Abstract

Summary: Association studies describe genetic associations between noncoding variants and disease susceptibility; however, they do not provide functional insight into the underlying molecular mechanisms of these variants. We present a protocol to assay the regulatory potential of thousands of noncoding variants using massively parallel reporter assays. We describe steps for oligo design, generating a plasmid pool, and extracting tag-seq libraries from cells to quantify the tested sequences.For complete details on the use and execution of this protocol, please refer to Oliveros and Delfosse et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Keywords

• Genetics
• Genomics
• High Throughput Screening
• Gene Expression
• Systems biology