Ciência Animal Brasileira (Jan 2015)

CENTRIFUGAÇÃO DE ESPERMATOZOIDES OVINOS EM GRADIENTE DE DENSIDADE CONTÍNUO: EFEITO SOBRE A TAXA DE PRENHEZ E PROPORÇÃO DO SEXO

  • Max Vitória Resende,
  • Carmo Emanuel Almeida Biscarde,
  • Laura Emília Panelli Martins,
  • Cláudia Kazumi Kiya,
  • Vera Fernanda Martins Hossepian de Lima,
  • Alberto Lopes Gusmão

DOI
https://doi.org/10.1590/1089-68916i124607
Journal volume & issue
Vol. 16, no. 1
pp. 125 – 132

Abstract

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The objective of this study was to evaluate the efficiency of continuous density gradients of Percoll and OptiPrep for separation of X-bearing sperm, and assess the influence on sperm viability, pregnancy rate and sex ratio. With this purpose 100 million spermatozoa were deposited into each tube containing density gradient, which were previously prepared in polystyrene tubes, 24 hours before centrifugation and stored at 4 °C. The tubes were centrifuged at 500xg for 15 minutes at 22 °C, and then the supernatants aspirated and sperm recovered from the bottom of the tubes. A sample was evaluated before and after centrifugation to verify motility and sperm vigor. The sperm were used for fixed time artificial insemination. The results indicated that centrifugation did not affect sperm viability and pregnancy rate, however there was no deviation of sex ratio for females. More studies are needed of adapting the centrifugation technique to sheep and thus obtain the deviation of sex ration as in bovine

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