Frontiers in Plant Science (Jul 2015)

Proteomic analysis and candidate allergenic proteins in Populus deltoides CL. ‘2KEN8’ mature pollen

  • Jin eZhang,
  • Jin eZhang,
  • Jin eZhang,
  • Li-Shuan eWu,
  • Li-Shuan eWu,
  • Li-Shuan eWu,
  • Wei eFan,
  • Xiao-Ling eZhang,
  • Hui-Xia eJia,
  • Hui-Xia eJia,
  • Yu eLi,
  • Ya-Fang eYin,
  • Ya-Fang eYin,
  • Jian-Jun eHu,
  • Jian-Jun eHu,
  • Meng-Zhu eLu,
  • Meng-Zhu eLu

DOI
https://doi.org/10.3389/fpls.2015.00548
Journal volume & issue
Vol. 6

Abstract

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Proteomic analysis was used to generate a map of Populus deltoides CL. ‘2KEN8’ mature pollen proteins. By applying 2-D electrophoresis, we resolved 403 protein spots from mature pollen. Using the matrix-assisted laser desorption/ionization time time-of-flight/time-of-flight tandem mass spectrometry method, we identified 178 distinct proteins from 218 protein spots expressed in mature pollen. Moreover, out of these, 28 proteins were identified as putative allergens. The expression patterns of these putative allergen genes indicate that several of these genes are highly expressed in pollen. In addition, the members of profilin allergen family were analyzed and their expression patterns were compared with their homologous genes in Arabidopsis and rice. Knowledge of these identified allergens has the potential to improve specific diagnosis and allergen immunotherapy treatment for patients with poplar pollen allergy.

Keywords