The structure of a 15-stranded actin-like filament from Clostridium botulinum

Fujiet Koh; Akihiro Narita; Lin Jie Lee; Kotaro Tanaka; Yong Zi Tan; Venkata P. Dandey; David Popp; Robert C. Robinson

doi:10.1038/s41467-019-10779-9

Nature Communications (Jun 2019)

The structure of a 15-stranded actin-like filament from Clostridium botulinum

Fujiet Koh,
Akihiro Narita,
Lin Jie Lee,
Kotaro Tanaka,
Yong Zi Tan,
Venkata P. Dandey,
David Popp,
Robert C. Robinson

Affiliations

Fujiet Koh: Institute of Molecular and Cell Biology, A*STAR (Agency for Science, Technology and Research), Biopolis
Akihiro Narita: Structural Biology Research Center, Graduate School of Science, Nagoya University
Lin Jie Lee: Institute of Molecular and Cell Biology, A*STAR (Agency for Science, Technology and Research), Biopolis
Kotaro Tanaka: Structural Biology Research Center, Graduate School of Science, Nagoya University
Yong Zi Tan: Department of Biochemistry and Molecular Biophysics, Columbia University
Venkata P. Dandey: National Resource for Automated Molecular Microscopy, Simons Electron Microscopy Center, New York Structural Biology Center
David Popp: Institute of Molecular and Cell Biology, A*STAR (Agency for Science, Technology and Research), Biopolis
Robert C. Robinson: Institute of Molecular and Cell Biology, A*STAR (Agency for Science, Technology and Research), Biopolis

DOI: https://doi.org/10.1038/s41467-019-10779-9
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 10

Abstract

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The plasmid-segregating actin-like protein ParM is encoded on the large, toxin carrying plasmid pCBH from Clostridium botulinum. Here the authors present the cryo-EM structure of the ParM filament that is formed from the association of 15 protofilaments and discuss its architecture.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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