Multidynamic Osteogenic Differentiation by Effective Polydopamine Micro-Arc Oxide Manipulations

Abstract

Yuqi Zhou,1,* Guifang Wang,2,* Tianqi Wang,1 Jiajia Wang,3 Xutao Wen,3 Haishui Sun,1 Lei Yu,1 Xiaoying Liu,4 Juanjuan Zhang,1 Qin Zhou,3 Yan Sun1 1School of Stomatology, Weifang Medical University, Weifang, People’s Republic of China; 2Department of Prosthodontics, Ninth People’s Hospital, Shanghai Jiaotong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai, People’s Republic of China; 3Department of Oral Surgery, Ninth People’s Hospital, Shanghai Jiaotong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai, People’s Republic of China; 4School of Bioscience and Technology, Weifang Medical University, Weifang, People’s Republic of China*These authors contributed equally to this workCorrespondence: Qin Zhou, Department of Oral Surgery, Ninth People’s Hospital, Shanghai Jiaotong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai, 200011, People’s Republic of China, Tel [phone], Email [email protected] Yan Sun, School of Stomatology, Weifang Medical University, Weifang, Shandong Province, 261053, People’s Republic of China, Tel [phone], Email [email protected]: The nanostructural modification of the oral implant surface can effectively mimic the morphology of natural bone tissue, allowing osteoblasts to achieve both proliferation and differentiation capabilities at the bone interface of the dental implant. To improve the osteoinductive activity on the surface of titanium implants for rapid osseointegration, we prepared a novel composite coating (MAO-PDA-NC) by micro-arc oxidation technique and immersion method and evaluated the proliferation, adhesion, and osteogenic differentiation of osteoblasts on this coating.Methods: The coatings were prepared by micro-arc oxidation (MAO) technique and immersion method, and characterized by scanning electron microscopy (SEM) and atomic force microscopy (AFM) for different coatings; the loading of PDA was examined using Fourier transform infrared spectroscopy (FTIR); the ion release capacity of the coatings was determined by inductively coupled plasma emission spectrometry (ICP-OES); the interfacial bonding of the coatings was examined using nanoscratch experiment strength. The cytotoxicity of the coating was examined by live/dead staining kit; cell proliferation viability was examined by CCK-8 kit; adhesion and osteogenic effect of the coating were examined by immunofluorescence staining and RT-PCR; osteogenic differentiation was examined by alkaline phosphatase staining.Results: The surface morphology of titanium implants was modified by micro-arc oxidation technology, and a new MAO-PDA-NC composite coating was successfully prepared. The results showed that the MAO-PDA-NC coating not only optimized the physical and chemical properties of the titanium implant surface but also significantly stimulated the biological properties of osteoblast adhesion, proliferation, and osteogenic differentiation on the coating surface.Conclusion: These results show that MAO-PDA-NC composite coating can significantly improve the surface properties of titanium implants and achieve a stable bond between implant and bone tissue, thus accelerating early osseointegration.Keywords: proliferation, differentiation, micro-arc oxidation, osseointegration

Keywords

• proliferation
• differentiation
• micro-arc oxidation
• osseointegration