Imaging of fluorescence anisotropy during photoswitching provides a simple readout for protein self-association

Namrata Ojha; Kristin H. Rainey; George H. Patterson

doi:10.1038/s41467-019-13843-6

Nature Communications (Jan 2020)

Imaging of fluorescence anisotropy during photoswitching provides a simple readout for protein self-association

Namrata Ojha,
Kristin H. Rainey,
George H. Patterson

Affiliations

Namrata Ojha: Section on Biophotonics, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health
Kristin H. Rainey: Section on Biophotonics, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health
George H. Patterson: Section on Biophotonics, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health

DOI: https://doi.org/10.1038/s41467-019-13843-6
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 11

Abstract

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Performing homo-FRET measurements in cells using a fluorescence microscope is challenging, especially when using high numerical aperture objective lenses. Here the authors present a method for improved homo-FRET measurements based on anisotropy changes in photoswitchable fluorescent proteins.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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