Amberlite XAD-4 is a convenient tool for removing Triton X-100 and Sarkosyl from protein solutions

Abstract

Amberlite has been shown to be an appropriate material for the adsorption of organic contaminants from aqueous solutions. In addition, Amberlite XAD-2 has been successfully used, as an alternative to Bio-Beads, to remove Triton X-100 from protein solutions, such as from samples of solubilized membrane proteins. However, Amberlite has not been tested as an adsorbent when a mixture of detergents is necessary to solubilize and refold a target protein. Here the authors show that Amberlite XAD-4 can be appropriately used to aid the purification process of proteins solubilized from inclusion bodies with the ternary detergent system consisting of Sarkosyl, Triton X-100 and CHAPS.

Keywords

• Amberlite XAD-2
• Amberlite XAD-4
• CHAPS
• Escherichia coli
• inclusion bodies
• N-lauroylsarcosine