Antioxidant activity of Phellinus igniarius fermentation mycelia contributions of different solvent extractions and their inhibitory effect on α-amylase
Yating Dong,
Tao Wang,
Bingcheng Gan,
Solomon P. Wasser,
Zhiyuan Zhang,
Jin Zhao,
Xinlian Duan,
Luping Cao,
Rencai Feng,
Renyun Miao,
Junjie Yan,
Zhi Wu
Affiliations
Yating Dong
Institute of Urban Agriculture, Chinese Academy of Agricultural Sciences, 36 Lazi East Road, Tianfu New Area, Chengdu, 610000, China; National Agricultural Science & Technology Center (NASC), 36 Lazi East Road, Tianfu New Area, Chengdu, 610000, China
Tao Wang
Institute of Urban Agriculture, Chinese Academy of Agricultural Sciences, 36 Lazi East Road, Tianfu New Area, Chengdu, 610000, China; National Agricultural Science & Technology Center (NASC), 36 Lazi East Road, Tianfu New Area, Chengdu, 610000, China
Bingcheng Gan
Institute of Urban Agriculture, Chinese Academy of Agricultural Sciences, 36 Lazi East Road, Tianfu New Area, Chengdu, 610000, China; National Agricultural Science & Technology Center (NASC), 36 Lazi East Road, Tianfu New Area, Chengdu, 610000, China; Corresponding author. Institute of Urban Agriculture, Chinese Academy of Agricultural Sciences, National Agricultural Science & Technology Center (NASC), 36 No. Lazi Road, Tianfu New Area, Chengdu, 610000, China.
Solomon P. Wasser
International Centre for Biotechnology and Biodiversity of Fungi, Institute of Evolution and Faculty of Natural Sciences, University of Haifa, Mt. Carmel, Haifa, 31905, Israel
Zhiyuan Zhang
Sichuan Academy of Agricultural Science, Institute of Agricultural Resources and Environment, SAAS, Institute of Edible Fungi, Shizishan Road NO. 4, Jinjiang District, Chengdu, 610066, China
Jin Zhao
Institute of Urban Agriculture, Chinese Academy of Agricultural Sciences, 36 Lazi East Road, Tianfu New Area, Chengdu, 610000, China; National Agricultural Science & Technology Center (NASC), 36 Lazi East Road, Tianfu New Area, Chengdu, 610000, China
Xinlian Duan
Institute of Urban Agriculture, Chinese Academy of Agricultural Sciences, 36 Lazi East Road, Tianfu New Area, Chengdu, 610000, China; National Agricultural Science & Technology Center (NASC), 36 Lazi East Road, Tianfu New Area, Chengdu, 610000, China
Luping Cao
Institute of Urban Agriculture, Chinese Academy of Agricultural Sciences, 36 Lazi East Road, Tianfu New Area, Chengdu, 610000, China; National Agricultural Science & Technology Center (NASC), 36 Lazi East Road, Tianfu New Area, Chengdu, 610000, China
Rencai Feng
Institute of Urban Agriculture, Chinese Academy of Agricultural Sciences, 36 Lazi East Road, Tianfu New Area, Chengdu, 610000, China; National Agricultural Science & Technology Center (NASC), 36 Lazi East Road, Tianfu New Area, Chengdu, 610000, China
Renyun Miao
Institute of Urban Agriculture, Chinese Academy of Agricultural Sciences, 36 Lazi East Road, Tianfu New Area, Chengdu, 610000, China; National Agricultural Science & Technology Center (NASC), 36 Lazi East Road, Tianfu New Area, Chengdu, 610000, China
Junjie Yan
Institute of Urban Agriculture, Chinese Academy of Agricultural Sciences, 36 Lazi East Road, Tianfu New Area, Chengdu, 610000, China; National Agricultural Science & Technology Center (NASC), 36 Lazi East Road, Tianfu New Area, Chengdu, 610000, China
Zhi Wu
Institute of Urban Agriculture, Chinese Academy of Agricultural Sciences, 36 Lazi East Road, Tianfu New Area, Chengdu, 610000, China; National Agricultural Science & Technology Center (NASC), 36 Lazi East Road, Tianfu New Area, Chengdu, 610000, China
Phellinus spp. have historically been used as traditional medicines to treat various diseases owing to their antioxidant, antitumor, and antidiabetic activities. Polysaccharides exhibit antidiabetic activity. In the present study, the polysaccharide contents of four Phellinus strains were compared. Phellinus igniarius QB72 possessed higher polysaccharide production, stronger 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, and α-amylase inhibitory activity. The three polysaccharides were sequentially extracted and partially purified from the fermentation mycelia using hot water, 1 % (NH4)2C2O4, and 1.25 M NaOH. Hot water extract polysaccharides exhibited higher DPPH radical scavenging and strong inhibitory activity against α-amylase with an IC50 value of 6.84 ± 0.37 mg/mL. The carbohydrate content of A1 (approximately 17457 Da) was approximately 88.28 %. The α-amylase inhibitory activity IC50 was decreased (3.178 ± 0.187 mg/mL) after DEAE water elution. P. igniarius QB72 hot-water extracts of partially purified polysaccharides have great potential as α-amylase inhibitors in food and medication-assisted additives.
