Journal of Extracellular Biology (Oct 2023)
Guidelines for the purification and characterization of extracellular vesicles of parasites
- Carmen Fernandez‐Becerra,
- Patrícia Xander,
- Daniel Alfandari,
- George Dong,
- Iris Aparici‐Herraiz,
- Irit Rosenhek‐Goldian,
- Mehrdad Shokouhy,
- Melisa Gualdron‐Lopez,
- Nicholy Lozano,
- Nuria Cortes‐Serra,
- Paula Abou Karam,
- Paula Meneghetti,
- Rafael Pedro Madeira,
- Ziv Porat,
- Rodrigo Pedro Soares,
- Adriana Oliveira Costa,
- Sima Rafati,
- Anabela‐Cordeiro da Silva,
- Nuno Santarém,
- Christopher Fernandez‐Prada,
- Marcel I. Ramirez,
- Dolores Bernal,
- Antonio Marcilla,
- Vera Lucia Pereira‐Chioccola,
- Lysangela Ronalte Alves,
- Hernando Del Portillo,
- Neta Regev‐Rudzki,
- Igor Correia deAlmeida,
- Sergio Schenkman,
- Martin Olivier,
- Ana Claudia Torrecilhas
Affiliations
- Carmen Fernandez‐Becerra
- ISGlobal, Barcelona Institute for Global Health Hospital Clínic‐Universitat de Barcelona Barcelona Spain
- Patrícia Xander
- Departamento de Ciências Farmacêuticas Laboratório de Imunologia Celular e Bioquímica de Fungos e Protozoários Departamento de Ciências Farmacêuticas Instituto de Ciências Ambientais Químicas e Farmacêuticas Universidade Federal de São Paulo (UNIFESP) São Paulo Brazil
- Daniel Alfandari
- Department of Biomolecular Sciences Weizmann Institute of Science (WIS) Rehovot Israel
- George Dong
- The Research Institute of the McGill University Health Centre McGill University Montréal Québec Canada
- Iris Aparici‐Herraiz
- ISGlobal, Barcelona Institute for Global Health Hospital Clínic‐Universitat de Barcelona Barcelona Spain
- Irit Rosenhek‐Goldian
- Department of Chemical Research Support WIS Rehovot Israel
- Mehrdad Shokouhy
- Department of Immunotherapy and Leishmania Vaccine Research Pasteur Institute of Iran Tehran Iran
- Melisa Gualdron‐Lopez
- ISGlobal, Barcelona Institute for Global Health Hospital Clínic‐Universitat de Barcelona Barcelona Spain
- Nicholy Lozano
- Departamento de Ciências Farmacêuticas Laboratório de Imunologia Celular e Bioquímica de Fungos e Protozoários Departamento de Ciências Farmacêuticas Instituto de Ciências Ambientais Químicas e Farmacêuticas Universidade Federal de São Paulo (UNIFESP) São Paulo Brazil
- Nuria Cortes‐Serra
- ISGlobal, Barcelona Institute for Global Health Hospital Clínic‐Universitat de Barcelona Barcelona Spain
- Paula Abou Karam
- Department of Biomolecular Sciences Weizmann Institute of Science (WIS) Rehovot Israel
- Paula Meneghetti
- Departamento de Ciências Farmacêuticas Laboratório de Imunologia Celular e Bioquímica de Fungos e Protozoários Departamento de Ciências Farmacêuticas Instituto de Ciências Ambientais Químicas e Farmacêuticas Universidade Federal de São Paulo (UNIFESP) São Paulo Brazil
- Rafael Pedro Madeira
- Departamento de Ciências Farmacêuticas Laboratório de Imunologia Celular e Bioquímica de Fungos e Protozoários Departamento de Ciências Farmacêuticas Instituto de Ciências Ambientais Químicas e Farmacêuticas Universidade Federal de São Paulo (UNIFESP) São Paulo Brazil
- Ziv Porat
- Flow Cytometry Unit Life Sciences Core Facilities, WIS Rehovot Israel
- Rodrigo Pedro Soares
- Instituto René Rachou/FIOCRUZ – MG Belo Horizonte Minas Gerais Brazil
- Adriana Oliveira Costa
- Departamento de Análises Clínicas e Toxicológicas Faculdade de Farmácia, Universidade Federal de Minas Gerais (UFMG) Belo Horizonte Minas Gerais Brasil
- Sima Rafati
- Department of Immunotherapy and Leishmania Vaccine Research Pasteur Institute of Iran Tehran Iran
- Anabela‐Cordeiro da Silva
- Host‐Parasite Interactions Group Institute of Research and Innovation in Health University of Porto Porto Portugal
- Nuno Santarém
- Host‐Parasite Interactions Group Institute of Research and Innovation in Health University of Porto Porto Portugal
- Christopher Fernandez‐Prada
- Faculty of Veterinary Medicine Université de Montréal Québec Canada
- Marcel I. Ramirez
- EVAHPI ‐ Extracellular Vesicles and Host‐Parasite Interactions Research Group Laboratório de Biologia Molecular e Sistemática de Tripanossomatideos Instituto Carlos Chagas‐Fiocruz Curitiba Paraná Brasil
- Dolores Bernal
- Departament de Bioquímica i Biologia Molecular, Facultat de Ciències Biològiques Universitat de València Burjassot Valencia Spain
- Antonio Marcilla
- Àrea de Parasitologia, Departament de Farmàcia i Tecnologia Farmacèutica i Parasitologia Universitat de València Burjassot Valencia Spain
- Vera Lucia Pereira‐Chioccola
- Laboratório de Biologia Molecular de Parasitas e Fungos, Centro de Parasitologia e Micologia Instituto Adolfo Lutz (IAL) São Paulo Brasil
- Lysangela Ronalte Alves
- Laboratório de Regulação da Expressão Gênica Instituto Carlos Chagas Fiocruz Paraná Curitiba Brazil
- Hernando Del Portillo
- ISGlobal, Barcelona Institute for Global Health Hospital Clínic‐Universitat de Barcelona Barcelona Spain
- Neta Regev‐Rudzki
- Department of Biomolecular Sciences Weizmann Institute of Science (WIS) Rehovot Israel
- Igor Correia deAlmeida
- Department of Biological Sciences Border Biomedical Research Center The University of Texas at El Paso El Paso Texas USA
- Sergio Schenkman
- Departamento de Microbiologia Imunologia e Parasitologia, UNIFESP São Paulo Brazil
- Martin Olivier
- The Research Institute of the McGill University Health Centre McGill University Montréal Québec Canada
- Ana Claudia Torrecilhas
- Departamento de Ciências Farmacêuticas Laboratório de Imunologia Celular e Bioquímica de Fungos e Protozoários Departamento de Ciências Farmacêuticas Instituto de Ciências Ambientais Químicas e Farmacêuticas Universidade Federal de São Paulo (UNIFESP) São Paulo Brazil
- DOI
- https://doi.org/10.1002/jex2.117
- Journal volume & issue
-
Vol. 2,
no. 10
pp. n/a – n/a
Abstract
Abstract Parasites are responsible for the most neglected tropical diseases, affecting over a billion people worldwide (WHO, 2015) and accounting for billions of cases a year and responsible for several millions of deaths. Research on extracellular vesicles (EVs) has increased in recent years and demonstrated that EVs shed by pathogenic parasites interact with host cells playing an important role in the parasite's survival, such as facilitation of infection, immunomodulation, parasite adaptation to the host environment and the transfer of drug resistance factors. Thus, EVs released by parasites mediate parasite‐parasite and parasite‐host intercellular communication. In addition, they are being explored as biomarkers of asymptomatic infections and disease prognosis after drug treatment. However, most current protocols used for the isolation, size determination, quantification and characterization of molecular cargo of EVs lack greater rigor, standardization, and adequate quality controls to certify the enrichment or purity of the ensuing bioproducts. We are now initiating major guidelines based on the evolution of collective knowledge in recent years. The main points covered in this position paper are methods for the isolation and molecular characterization of EVs obtained from parasite‐infected cell cultures, experimental animals, and patients. The guideline also includes a discussion of suggested protocols and functional assays in host cells
Keywords