Spore viability of fern species of the genera Amauropelta, Blechnum, and Physematium from central Argentina after low temperature storage

Abstract

Background and aims: Ferns are sensitive to environmental changes, inhabiting ecosystems susceptible to degradation. Spore banks and in vitro spore culture are useful tools employed in their conservation. The objective was to test the ability of the spores to germinate after being stored in a freezer and to develop gametophytes and sporophytes, in native fern species of genera Amauropelta, Blechnum and Physematium. M&M: Spores of A. argentina, B. auriculatum and P. montevidensis were stored at -20 ºC in dry conditions for 6 and 12 months. They were sown in vitro in Dyer liquid medium and incubated in a growth chamber. The means of germination percentages were calculated and a one-way Student’s t-test was employed. Gametophyte and sporophyte development was registered under light and stereoscopic microscopes. Results: Depending on the species, statistical differences were recorded in germination percentages (viability) between both storage periods. The spores of A. argentina kept in freezer for 12 months completely lost viability. In B. auriculatum, viability decreased over time, and in P. montevidensis it remained constant. Gametophytes and sporophytes developed in all cultures; except in A. argentina spores, with 12 months in freezer. Conclusions: The viability of the spores under dry storage at low temperature could be conditioned by the ecological requirements of the species. Protocols used for spore storage and culture allowed obtaining gametophytes and sporophytes in trials.

Keywords

• Amauropelta
• Blechnum
• ex-situ conservation
• fern, freezing
• Physematium
• spore longevity