Phenolic composition and bioactivity of Ribes magellanicum fruits from southern Patagonia
Alberto Burgos-Edwards,
Cristina Theoduloz,
Sophia Miño,
Debasish Ghosh,
Vladimir Shulaev,
Crister Ramírez,
Laura Sánchez-Jardón,
Ricardo Rozzi,
Guillermo Schmeda-Hirschmann
Affiliations
Alberto Burgos-Edwards
Laboratorio de Química de Productos Naturales, Instituto de Química de Recursos Naturales, Campus Lircay, Universidad de Talca, 3480094, Talca, Chile; Centro de Estudios en Alimentos Procesados (CEAP), CONICYT-Regional, Gore Maule R0912001, 3480094, Talca, Chile
Cristina Theoduloz
Laboratorio de Cultivo Celular, Facultad de Ciencias de la Salud, Universidad de Talca, Campus Lircay, 3480094, Talca, Chile; Cape Horn International Center (CHIC), O’Higgins 310, 6350000, Puerto Williams, Chile
Sophia Miño
Laboratorio de Química de Productos Naturales, Instituto de Química de Recursos Naturales, Campus Lircay, Universidad de Talca, 3480094, Talca, Chile; Centro de Estudios en Alimentos Procesados (CEAP), CONICYT-Regional, Gore Maule R0912001, 3480094, Talca, Chile
Debasish Ghosh
Department of Biological Sciences and Advanced Environmental Research Institute, The University of North Texas, Denton, TX, 76203, USA
Vladimir Shulaev
Department of Biological Sciences and Advanced Environmental Research Institute, The University of North Texas, Denton, TX, 76203, USA
Crister Ramírez
Cape Horn International Center (CHIC), O’Higgins 310, 6350000, Puerto Williams, Chile
Laura Sánchez-Jardón
Cape Horn International Center (CHIC), O’Higgins 310, 6350000, Puerto Williams, Chile; Centro de Investigación GAIA Antártica (CIGA), Universidad de Magallanes, Av. Bulnes 1890, 6200000, Punta Arenas, Chile
Ricardo Rozzi
Cape Horn International Center (CHIC), O’Higgins 310, 6350000, Puerto Williams, Chile; Centro Universitario Cabo de Hornos, Universidad de Magallanes, Teniente Muñoz 166, 6350000, Puerto Williams, Chile
Guillermo Schmeda-Hirschmann
Laboratorio de Química de Productos Naturales, Instituto de Química de Recursos Naturales, Campus Lircay, Universidad de Talca, 3480094, Talca, Chile; Cape Horn International Center (CHIC), O’Higgins 310, 6350000, Puerto Williams, Chile; Corresponding author. Laboratorio de Química de Productos Naturales, Instituto de Química de Recursos Naturales, Campus Lircay, Universidad de Talca, 3480094, Talca, Chile.
Eight Ribes magellanicum collections from three different places in southern Patagonia were compared for content of different groups of phenolics, antioxidant capacity and inhibition of enzymes related to metabolic syndrome (α-amylase, α-glucosidase and pancreatic lipase). The sample with the highest antioxidant capacity was assessed for glutathione (GSH) synthesis stimulation in human gastric adenocarcinoma (AGS) cells. The chemical profile was determined by high performance liquid chromatography with tandem mass spectrometry detection (HPLC-MS/MS) and the main phenolics were quantified. The samples from Navarino Island and Reserva Nacional Magallanes showed higher content of anthocyanins and caffeoylquinic acid, with better activity towards α-glucosidase and antioxidant capacity. A sample from Omora (Navarino Island), significantly increased intracellular GSH content in AGS cells. Some 70 compounds were identified in the fruit extracts by HPLC-MS/MS. The glucoside and rutinoside from delphinidin and cyanidin and 3-caffeoylquinic acid were the main compounds. Different chemical profiles were found according to the collection places.
