Prevalence and characteristics of multidrug-resistant Proteus mirabilis from broiler farms in Shandong Province, China

Zixuan Li; Chong Peng; Gerui Zhang; Yuanyu Shen; Yuxuan Zhang; Cong Liu; Mengda Liu; Fangkun Wang

Poultry Science (Apr 2022)

Prevalence and characteristics of multidrug-resistant Proteus mirabilis from broiler farms in Shandong Province, China

Zixuan Li,
Chong Peng,
Gerui Zhang,
Yuanyu Shen,
Yuxuan Zhang,
Cong Liu,
Mengda Liu,
Fangkun Wang

Affiliations

Zixuan Li: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Shandong, 271018, PR China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, Tai'an 271018, Shandong, PR China
Chong Peng: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Shandong, 271018, PR China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, Tai'an 271018, Shandong, PR China
Gerui Zhang: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Shandong, 271018, PR China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, Tai'an 271018, Shandong, PR China
Yuanyu Shen: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Shandong, 271018, PR China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, Tai'an 271018, Shandong, PR China
Yuxuan Zhang: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Shandong, 271018, PR China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, Tai'an 271018, Shandong, PR China
Cong Liu: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Shandong, 271018, PR China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, Tai'an 271018, Shandong, PR China
Mengda Liu: Laboratory of Zoonoses, China Animal Health and Epidemiology Center, Qingdao, Shandong, 266032, PR China
Fangkun Wang: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Shandong, 271018, PR China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, Tai'an 271018, Shandong, PR China; Corresponding author:

Journal volume & issue: Vol. 101, no. 4
p. 101710

Abstract

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ABSTRACT: Animal-derived Proteus mirabilis (P. mirabilis) is an important food-borne zoonotic bacillus and widely exists in the broiler-breeding industry. The present study was designed to explore the P. mirabilis prevalence and antimicrobial resistance characteristics in 6 conventional broiler-fattening farms in Shandong Province, China. The overall isolation rate of P. mirabilis was 7.07% (50/707). Antimicrobial resistance was very common in the P. mirabilis isolated from these farms and varied for different antibacterial drugs, with chloramphenicol, ciprofloxacin, and trimethoprim-sulfamethoxazole having the highest resistance rate (98%) and aztreonam the lowest (0%). Multidrug resistance was as high as 100%. The majority of the MDR isolates were resistant to between 9 and 12 of the antibiotics, with these accounting for 76% (38/50) of multidrug resistant strains. These P. mirabilis isolates carried 24 drug-resistance genes in 6 types, with stcM having the highest rate (96%) and cmlA, blaTEM, and qnrC the lowest (2%). Superdrug resistance gene blaNDM-1 was found in 10% (5/50) of isolates from poultry farms in Shandong. All the P. mirabilis isolates carried at least 6 virulence genes, with 100% detection rates of the ireA and hpmA genes. Our study revealed that the P. mirabilis strains isolated in the Shandong area all showed the MDR phenotype and the poultry-derived carbapenem-resistant MDR P. mirabilis strains may pose a potential risk to humans. Surveillance findings presented herein will be conducive to our understanding of the prevalence and characteristics of carbapenem-resistant P. mirabilis strains in Shandong, China.

Published in Poultry Science

ISSN: 0032-5791 (Print); 1525-3171 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Agriculture: Animal culture
Website: https://www.journals.elsevier.com/poultry-science

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