One-step construction of circularized nanodiscs using SpyCatcher-SpyTag

Shanwen Zhang; Qian Ren; Scott J. Novick; Timothy S. Strutzenberg; Patrick R. Griffin; Huan Bao

doi:10.1038/s41467-021-25737-7

Nature Communications (Sep 2021)

One-step construction of circularized nanodiscs using SpyCatcher-SpyTag

Shanwen Zhang,
Qian Ren,
Scott J. Novick,
Timothy S. Strutzenberg,
Patrick R. Griffin,
Huan Bao

Affiliations

Shanwen Zhang: Department of Molecular Medicine, The Scripps Research Institute
Qian Ren: Department of Molecular Medicine, The Scripps Research Institute
Scott J. Novick: Department of Molecular Medicine, The Scripps Research Institute
Timothy S. Strutzenberg: Department of Molecular Medicine, The Scripps Research Institute
Patrick R. Griffin: Department of Molecular Medicine, The Scripps Research Institute
Huan Bao: Department of Molecular Medicine, The Scripps Research Institute

DOI: https://doi.org/10.1038/s41467-021-25737-7
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 9

Abstract

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Circularised nanodiscs (cNDs) are able to stabilise large lipid bilayer patches and are used for structural and functional studies. Current techniques to build cNDs have numerous steps and low yields; here the authors report a single step construction method using the SpyCatcher-SpyTag system.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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