Genes (Jun 2019)
The Genome of the Steller Sea Lion (<i>Eumetopias jubatus</i>)
- Harwood H. Kwan,
- Luka Culibrk,
- Gregory A. Taylor,
- Sreeja Leelakumari,
- Ryan Tan,
- Shaun D. Jackman,
- Kane Tse,
- Tina MacLeod,
- Dean Cheng,
- Eric Chuah,
- Heather Kirk,
- Pawan Pandoh,
- Rebecca Carlsen,
- Yongjun Zhao,
- Andrew J. Mungall,
- Richard Moore,
- Inanc Birol,
- Marco A. Marra,
- David A.S. Rosen,
- Martin Haulena,
- Steven J. M. Jones
Affiliations
- Harwood H. Kwan
- Canada’s Michael Smith Genome Sciences Centre, British Columbia Cancer, Vancouver, BC V5Z-4S6, Canada
- Luka Culibrk
- Canada’s Michael Smith Genome Sciences Centre, British Columbia Cancer, Vancouver, BC V5Z-4S6, Canada
- Gregory A. Taylor
- Canada’s Michael Smith Genome Sciences Centre, British Columbia Cancer, Vancouver, BC V5Z-4S6, Canada
- Sreeja Leelakumari
- Canada’s Michael Smith Genome Sciences Centre, British Columbia Cancer, Vancouver, BC V5Z-4S6, Canada
- Ryan Tan
- Canada’s Michael Smith Genome Sciences Centre, British Columbia Cancer, Vancouver, BC V5Z-4S6, Canada
- Shaun D. Jackman
- Canada’s Michael Smith Genome Sciences Centre, British Columbia Cancer, Vancouver, BC V5Z-4S6, Canada
- Kane Tse
- Canada’s Michael Smith Genome Sciences Centre, British Columbia Cancer, Vancouver, BC V5Z-4S6, Canada
- Tina MacLeod
- Canada’s Michael Smith Genome Sciences Centre, British Columbia Cancer, Vancouver, BC V5Z-4S6, Canada
- Dean Cheng
- Canada’s Michael Smith Genome Sciences Centre, British Columbia Cancer, Vancouver, BC V5Z-4S6, Canada
- Eric Chuah
- Canada’s Michael Smith Genome Sciences Centre, British Columbia Cancer, Vancouver, BC V5Z-4S6, Canada
- Heather Kirk
- Canada’s Michael Smith Genome Sciences Centre, British Columbia Cancer, Vancouver, BC V5Z-4S6, Canada
- Pawan Pandoh
- Canada’s Michael Smith Genome Sciences Centre, British Columbia Cancer, Vancouver, BC V5Z-4S6, Canada
- Rebecca Carlsen
- Canada’s Michael Smith Genome Sciences Centre, British Columbia Cancer, Vancouver, BC V5Z-4S6, Canada
- Yongjun Zhao
- Canada’s Michael Smith Genome Sciences Centre, British Columbia Cancer, Vancouver, BC V5Z-4S6, Canada
- Andrew J. Mungall
- Canada’s Michael Smith Genome Sciences Centre, British Columbia Cancer, Vancouver, BC V5Z-4S6, Canada
- Richard Moore
- Canada’s Michael Smith Genome Sciences Centre, British Columbia Cancer, Vancouver, BC V5Z-4S6, Canada
- Inanc Birol
- Canada’s Michael Smith Genome Sciences Centre, British Columbia Cancer, Vancouver, BC V5Z-4S6, Canada
- Marco A. Marra
- Canada’s Michael Smith Genome Sciences Centre, British Columbia Cancer, Vancouver, BC V5Z-4S6, Canada
- David A.S. Rosen
- Institute for the Oceans and Fisheries, University of British Columbia, Vancouver, BC V6T-1Z4, Canada
- Martin Haulena
- Vancouver Aquarium, Vancouver, BC V6G 3E2, Canada
- Steven J. M. Jones
- Canada’s Michael Smith Genome Sciences Centre, British Columbia Cancer, Vancouver, BC V5Z-4S6, Canada
- DOI
- https://doi.org/10.3390/genes10070486
- Journal volume & issue
-
Vol. 10,
no. 7
p. 486
Abstract
The Steller sea lion is the largest member of the Otariidae family and is found in the coastal waters of the northern Pacific Rim. Here, we present the Steller sea lion genome, determined through DNA sequencing approaches that utilized microfluidic partitioning library construction, as well as nanopore technologies. These methods constructed a highly contiguous assembly with a scaffold N50 length of over 14 megabases, a contig N50 length of over 242 kilobases and a total length of 2.404 gigabases. As a measure of completeness, 95.1% of 4104 highly conserved mammalian genes were found to be complete within the assembly. Further annotation identified 19,668 protein coding genes. The assembled genome sequence and underlying sequence data can be found at the National Center for Biotechnology Information (NCBI) under the BioProject accession number PRJNA475770.
Keywords