Zhongguo youzhi (Jun 2024)
奇数链脂肪酸抑制脂多糖诱导的Caco-2 细胞炎症反应研究Protective effect of odd-chain fatty acids on lipopolysaccharide- induced inflammation in Caco-2 cells
Abstract
为明确奇数链脂肪酸（OCFA）与人体健康的关系，采用气相色谱-质谱法（GC-MS）检测分析健康人群和结肠癌患者血清中OCFA的分布特征差异，并采用脂多糖（LPS）诱导的Caco-2细胞炎症模型探究OCFA对炎症的抑制效应，包括对细胞活性、促炎因子基因表达以及脂肪酸组成的影响。结果表明：健康人群中的OCFA（C15∶ 0和C17∶ 0）含量显著高于结肠癌人群；男性以及高龄（59岁以上）结肠癌人群中C15∶ 0和C17∶ 0水平偏低；C15∶ 0和C17∶ 0对细胞活力无影响，C15∶ 0能够显著降低LPS刺激后Caco-2细胞中促炎因子TNF-α、IL-8和iNOS的表达，C17∶ 0能够显著降低促炎因子IL-6、TNF-α、IL-8和iNOS的表达，两种OCFA均能够起到类似于n-3 PUFA缓解炎症刺激的作用；两种OCFA（C15∶ 0和C17∶ 0）能够在肠道细胞Caco-2中吸收并代谢成C17∶ 0、C17∶ 1。综上，OCFA能够调节肠道炎症因子的表达进而抑制肠上皮细胞炎症反应，对肠道健康有潜在的保护作用。In order to explore the health effects of odd-chain fatty acids (OCFA), serum OCFA levels in the healthy individuals and colon cancer patients were measured by gas chromatography-mass spectrometry (GC-MS) to investigate the differences in the distribution characteristics, and the protective effects of OCFA on inflammation by using the lipopolysaccharide (LPS)-induced Caco-2 cell inflammation model were studied, including the changes in cell activity, pro-inflammatory factor gene expression and fatty acid composition. The results showed that the levels of two main OCFA (C15∶ 0 and C17∶ 0) in healthy individuals were significantly higher than that in colon cancer patients.The levels of C15∶ 0 and C17∶ 0 in male patients and patients over the age of 59 were significantly lower. C15∶ 0 and C17∶ 0 had no effect on cell activity. C15∶ 0 could significantly inhibit the expression of pro-inflammatory factors TNF-α, IL-8 and iNOS in Caco-2 cells with LPS stimulation, C17∶ 0 could significantly reduce the expression of IL-6, TNF-α, IL-8 and iNOS. Two main OCFA could play a similar role in alleviating inflammatory stimulation as n-3 PUFA. Two main OCFA could be easily incorporated by Caco-2 cells into C17∶ 0 and C17∶ 1 in Caco-2. In conclusion, OCFA may have a potential preventive effect on inflammation and intestinal health by regulating the expression of pro-inflammatory cytokines.
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