Cryo-EM structures of the translocational binary toxin complex CDTa-bound CDTb-pore from Clostridioides difficile

Abstract

Binary toxin translocation system consists of an enzymatic subunit and translocation pore. Here, the authors report the high-resolution cryo-EM structure of Clostridioides difficile binary toxin, comprising ADP-ribosyltransferase CDTa bound to the CDTb pore and revealing translocational unfolding of CDTa in the CDTb-pore.