The alteration of NK cells phenotypes related to the functions and dengue disease outcomes

Napas Taechasan; Iris Scherwitzl; Piyada Supasa; Wanwisa Dejnirattisai; Kanokwan Sriruksa; Wannee Limpitikul; Prida Malasit; Gavin R Screaton; Juthathip Mongkolsapaya; Thaneeya Duangchinda

Virus Research (Jul 2024)

The alteration of NK cells phenotypes related to the functions and dengue disease outcomes

Napas Taechasan,
Iris Scherwitzl,
Piyada Supasa,
Wanwisa Dejnirattisai,
Kanokwan Sriruksa,
Wannee Limpitikul,
Prida Malasit,
Gavin R Screaton,
Juthathip Mongkolsapaya,
Thaneeya Duangchinda

Affiliations

Napas Taechasan: Department of Immunology, Graduate Program in Immunology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand; Department of Research and Development, Division of Dengue Hemorrhagic Fever Research, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok-Noi, Bangkok 10700, Thailand
Iris Scherwitzl: Department of Immunology, Graduate Program in Immunology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand; Department of Research and Development, Division of Dengue Hemorrhagic Fever Research, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok-Noi, Bangkok 10700, Thailand; Nuffield Department of Medicine, Wellcome Centre for Human Genetics, University of Oxford, Oxford, UK; Division of Emerging Infectious Disease, Research Department, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok-Noi, Bangkok 10700, Thailand; Pediatric Department, Khon Kaen Hospital, Ministry of Public Health, Khon Kaen 40000, Thailand; Pediatric Department, Songkhla Hospital, Ministry of Public Health, Songkhla 90100, Thailand; Molecular Biology of Dengue and Flaviviruses Research Team, Medical Molecular Biotechnology Research Group, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Bangkok 12120, Thailand; Chinese Academy of Medical Science (CAMS) Oxford Institute (COI), University of Oxford, Oxford, UK; Mahidol-Oxford Tropical Medicine Research Unit, Bangkok, Thailand; Department of Medicine, University of Oxford, Oxford, UK; Department of Medicine, Faculty of Medicine, Imperial College, Hammersmith Campus, London W12 0NN, UK
Piyada Supasa: Nuffield Department of Medicine, Wellcome Centre for Human Genetics, University of Oxford, Oxford, UK
Wanwisa Dejnirattisai: Nuffield Department of Medicine, Wellcome Centre for Human Genetics, University of Oxford, Oxford, UK; Division of Emerging Infectious Disease, Research Department, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok-Noi, Bangkok 10700, Thailand
Kanokwan Sriruksa: Pediatric Department, Khon Kaen Hospital, Ministry of Public Health, Khon Kaen 40000, Thailand
Wannee Limpitikul: Pediatric Department, Songkhla Hospital, Ministry of Public Health, Songkhla 90100, Thailand
Prida Malasit: Department of Research and Development, Division of Dengue Hemorrhagic Fever Research, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok-Noi, Bangkok 10700, Thailand; Molecular Biology of Dengue and Flaviviruses Research Team, Medical Molecular Biotechnology Research Group, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Bangkok 12120, Thailand
Gavin R Screaton: Nuffield Department of Medicine, Wellcome Centre for Human Genetics, University of Oxford, Oxford, UK; Chinese Academy of Medical Science (CAMS) Oxford Institute (COI), University of Oxford, Oxford, UK
Juthathip Mongkolsapaya: Nuffield Department of Medicine, Wellcome Centre for Human Genetics, University of Oxford, Oxford, UK; Chinese Academy of Medical Science (CAMS) Oxford Institute (COI), University of Oxford, Oxford, UK; Mahidol-Oxford Tropical Medicine Research Unit, Bangkok, Thailand; Department of Medicine, University of Oxford, Oxford, UK
Thaneeya Duangchinda: Department of Research and Development, Division of Dengue Hemorrhagic Fever Research, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok-Noi, Bangkok 10700, Thailand; Molecular Biology of Dengue and Flaviviruses Research Team, Medical Molecular Biotechnology Research Group, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Bangkok 12120, Thailand; Corresponding author at: Molecular Biology of Dengue and Flavivirus Research Team, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani 12120, Thailand.

Journal volume & issue: Vol. 345
p. 199382

Abstract

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Natural killer cells (NK cells) are the front line of immune cells to combat pathogens and able to influence the subsequent adaptive immune responses. One of the factors contributing to pathogenesis in dengue hemorrhagic fever (DHF) disease is aberrant immune activation during early phase of infection. This study explored the profile of NK cells in dengue infected pediatric patients with different degrees of disease severity. DHF patients contained higher frequency of activated NK cells but lower ratio of CD56dim:CD56bright NK subsets. Activated NK cells exhibited alterations in several NK receptors. Interestingly, the frequencies of NKp30 expressing activated NK cells were more pronounced in dengue fever (DF) than in DHF pediatric patients. In vitro functional analysis indicated that degranulation of NK cells in responding to dengue infected dendritic cells (DCs) required cell-cell contact and type I IFNs. Meanwhile, Interferon gamma (IFN-γ) production initially required cell-cell contact and type I IFNs followed by Interleukin-12 (IL-12), Interleukin-15 (IL-15) and Interleukin-18 (IL-18) resulting in the amplification of IFN-γ producing NK cells over time. This study highlighted the complexity and the factors influencing NK cells responses to dengue virus. Degree of activation, phenotypes of activated cells and the crosstalk between NK cells and other immune cells, could modulate the outcome of NK cells function in the dengue disease.

Published in Virus Research

ISSN: 1872-7492 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Microbiology; Medicine: Internal medicine: Infectious and parasitic diseases
Website: https://www.sciencedirect.com/journal/virus-research

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