Human Vaccines & Immunotherapeutics (Dec 2018)

A surrogate assay for measuring Coxsackievirus A6 neutralizing antibodies

  • Yao Su,
  • Pan Chen,
  • Fan Gao,
  • Lianlian Bian,
  • Shiyang Sun,
  • Fangyu Dong,
  • Yalin Hu,
  • Qunying Mao,
  • Wei Jiang,
  • Xing Wu,
  • Zhenglun Liang

DOI
https://doi.org/10.1080/21645515.2018.1504540
Journal volume & issue
Vol. 14, no. 12
pp. 3034 – 3040

Abstract

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Coxsackievirus A6 (CV-A6) is one of pathogens causing hand, foot and mouth disease (HFMD) and becomes a new challenge to HFMD control. In this study, we first built a single-round pseudovirus infection system for CV-A6, and then developed a pseudovirus luciferase assay (PVLA) for anti-CV-A6 neutralizing antibody (NtAb) quantification. Since cytopahtic effect (CPE) is considered as the gold standard test for anti-enterovirus NtAb detection, a comparison study has been performed using 318 clinical serum samples, as measured both by PVLA and CPE. The sensitivity and specificity of PVLA was 94.9% (95% CI between 90.8–97.5%) and 92.7% (95% CI between 86.6–96.6%), respectively. Statistical analysis revealed that PVLA and CPE were highly correlated (spearman r = 0.931, P < 0.0001) and in good agreement (94.0%, 95% CI between 90.8–96.4%), showing that PVLA could be used as a surrogate assay for anti-CV-A6 NtAb detection and served as a valuable tool for CV-A6 vaccine evaluation and CV-A6 epidemiological surveillance.

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