Evaluation of SRAP markers efficiency in genetic diversity of Aspergillus flavus from peanut-cropped soils in China

Abstract

In order to evaluation the efficiency of SRAP markers on genetic diversity of Aspergillus flavus, we screened out 17 sets of primer pairs which could produce clear and reproducible SRAP bands from 150 SRAP primer pairs. The size of SRAP fragments ranged from 120 to 2100 bp. Primer pair Me10/Em9 produced the maximum number of polymorphic bands (12 bands), while Me8/Em13 produced the fewest number of polymorphic bands (only 1). Through analysis genetic diversity ability of different sets of primer pairs, the set of 12 primer pairs was selected for SRAP genetic marker of A. flavus. Cluster analysis was performed based on the genetic similarity coefficients, which ranged from 0.53 to 0.89. A dendrogram assembled using the unweighted pair-group method with arithmetic averages grouped A. flavus samples into 5 main clusters. The results suggested that SRAP marker is a useful molecular technology for the diversity of A. flavus from peanut soils in China.

Keywords

• Sequence-related amplified polymorphism
• Genetic diversity
• Aspergillus flavus
• Peanut
• Primer screening