Constituents of <em>Chamaecrista diphylla</em> (L.) Greene Leaves with Potent Antioxidant Capacity: A Feature-Based Molecular Network Dereplication Approach
Paulo Gomes,
Luis Quirós-Guerrero,
Abraão Muribeca,
José Reis,
Sônia Pamplona,
Anderson H. Lima,
Mariele Trindade,
Consuelo Silva,
Jesus N. S. Souza,
Jean A. Boutin,
Jean-Luc Wolfender,
Milton Silva
Affiliations
Paulo Gomes
Laboratory of Liquid Chromatography (Labcrol), Institute of Exact and Natural Sciences, Federal University of Pará, Belém 66075-110, Brazil
Luis Quirós-Guerrero
School of Pharmaceutical Sciences, University de Geneva, CMU, Rue Michel-Servet 1, CH-1206 Geneva, Switzerland
Abraão Muribeca
Laboratory of Liquid Chromatography (Labcrol), Institute of Exact and Natural Sciences, Federal University of Pará, Belém 66075-110, Brazil
José Reis
Laboratory of Liquid Chromatography (Labcrol), Institute of Exact and Natural Sciences, Federal University of Pará, Belém 66075-110, Brazil
Sônia Pamplona
Laboratory of Liquid Chromatography (Labcrol), Institute of Exact and Natural Sciences, Federal University of Pará, Belém 66075-110, Brazil
Anderson H. Lima
Drug Planning and Development Laboratory, Federal University of Pará, Belém 66075-110, Brazil
Mariele Trindade
Faculty of Food Engineering, Federal University of Pará, Belém 66075-110, Brazil
Consuelo Silva
Laboratory of Liquid Chromatography (Labcrol), Institute of Exact and Natural Sciences, Federal University of Pará, Belém 66075-110, Brazil
Jesus N. S. Souza
Faculty of Food Engineering, Federal University of Pará, Belém 66075-110, Brazil
Jean A. Boutin
PHARMADEV (Pharmacochimie et Biologie pour le Développement), Université Toulouse 3 Paul Sabatier, Faculté de Pharmacie, CEDEX 9, 31062 Toulouse, France
Jean-Luc Wolfender
School of Pharmaceutical Sciences, University de Geneva, CMU, Rue Michel-Servet 1, CH-1206 Geneva, Switzerland
Milton Silva
Laboratory of Liquid Chromatography (Labcrol), Institute of Exact and Natural Sciences, Federal University of Pará, Belém 66075-110, Brazil
Chamaecrista diphylla (L.) Greene (Fabaceae/Caesalpiniaceae) is a herbaceous plant that is widely distributed throughout the Americas. Plants from this genus have been used in traditional medicine as a laxative, to heal wounds, and to treat ulcers, snake and scorpion bites. In the present study, we investigated the chemical composition of Chamaecrista diphylla leaves through a mass spectrometry molecular network approach. The oxygen radical absorbance capacity (ORAC) for the ethanolic extract, enriched fractions and isolated compounds was assessed. Overall, thirty-five compounds were annotated for the first time in C. diphylla. Thirty-two of them were reported for the first time in the genus. The isolated compounds 9, 12, 24 and 33 showed an excellent antioxidant capacity, superior to the extract and enriched fractions. Bond dissociation energy calculations were performed to explain and sustain the antioxidant capacity found. According to our results, the leaves of C. diphylla represent a promising source of potent antioxidant compounds.