Integration-free induced pluripotent stem cell line derived from a 62-years-old male donor with APOE-epsilon4/epsilon4 alleles

Ruiyun Guo; Xin Liu; Jinyu Zhang; Xiaowei Ma; Dongyun Gou; Zhenhuan Ma; Lei Wang; Jun Ma; Huixian Cui

Stem Cell Research (May 2022)

Integration-free induced pluripotent stem cell line derived from a 62-years-old male donor with APOE-epsilon4/epsilon4 alleles

Ruiyun Guo,
Xin Liu,
Jinyu Zhang,
Xiaowei Ma,
Dongyun Gou,
Zhenhuan Ma,
Lei Wang,
Jun Ma,
Huixian Cui

Affiliations

Ruiyun Guo: Hebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Province 050017, China
Xin Liu: Hebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Province 050017, China
Jinyu Zhang: Hebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Province 050017, China
Xiaowei Ma: Hebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Province 050017, China; Department of Neurology, The First Hospital of Hebei Medical University, Hebei Province 050017, China
Dongyun Gou: Hebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Province 050017, China; Department of Neurology, The First Hospital of Hebei Medical University, Hebei Province 050017, China
Zhenhuan Ma: Hebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Province 050017, China
Lei Wang: Human Anatomy Department, Hebei Medical University, Hebei Province 050017, China
Jun Ma: Hebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Province 050017, China; Human Anatomy Department, Hebei Medical University, Hebei Province 050017, China; Corresponding author at: Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China.
Huixian Cui: Hebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Province 050017, China; Human Anatomy Department, Hebei Medical University, Hebei Province 050017, China

Journal volume & issue: Vol. 61
p. 102746

Abstract

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The ε4 allele of the Apolipoprotein E gene (APOE4) continues to be the strongest genetic risk factor associated with sporadic Alzheimer’s disease since its discovery compared to the most common ε3 allele. Nevertheless, there is a lack of APOE4-mutant human neuronal models in vitro or in vivo. Hence, we presented an iPSC line of an APOE-ε4/ε4 alleles carrier, a male donor suffering from Alzheimer’s disease combined with cerebral infarction. The established iPSC line showed standard characteristics of pluripotency. Collectively, the present study provides a useful resource to reveal the phenotype and explore the mechanism of APOE4 related Alzheimer’s disease.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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