Carbon dots (CDs) derived from chitosan via a solvothermal method were covalentlylinked to an indium tin oxide (ITO) surface and showed a direct photoelectrochemical response.We attribute the photocurrent of the ITO-silane-CD surface to a photogenerated electron-transferprocess by CDs under illumination. The ITO-silane-CD surface was successfully used forac-photocurrent imaging. This opens up new applications for CDs as biocompatible andlight-addressable electrochemical sensors in bioimaging applications.