Journal of Veterinary Research (May 2020)
A novel, rapid, and simple PMA-qPCR method for detection and counting of viable Brucella organisms
- Zhang Shi-Jun,
- Wang Lu-Lu,
- Lu Shi-Ying,
- Hu Pan,
- Li Yan-Song,
- Zhang Ying,
- Chang Heng-Zhen,
- Zhai Fei-Fei,
- Liu Zeng-Shan,
- Li Zhao-Hui,
- Ren Hong-Lin
Affiliations
- Zhang Shi-Jun
- Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, College of Animal Sciences, Jilin University, Xi An Da Lu 5333, Changchun130062, China
- Wang Lu-Lu
- Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, College of Animal Sciences, Jilin University, Xi An Da Lu 5333, Changchun130062, China
- Lu Shi-Ying
- Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, College of Animal Sciences, Jilin University, Xi An Da Lu 5333, Changchun130062, China
- Hu Pan
- Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, College of Animal Sciences, Jilin University, Xi An Da Lu 5333, Changchun130062, China
- Li Yan-Song
- Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, College of Animal Sciences, Jilin University, Xi An Da Lu 5333, Changchun130062, China
- Zhang Ying
- Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, College of Animal Sciences, Jilin University, Xi An Da Lu 5333, Changchun130062, China
- Chang Heng-Zhen
- Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, College of Animal Sciences, Jilin University, Xi An Da Lu 5333, Changchun130062, China
- Zhai Fei-Fei
- Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, College of Animal Sciences, Jilin University, Xi An Da Lu 5333, Changchun130062, China
- Liu Zeng-Shan
- Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, College of Animal Sciences, Jilin University, Xi An Da Lu 5333, Changchun130062, China
- Li Zhao-Hui
- Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, College of Animal Sciences, Jilin University, Xi An Da Lu 5333, Changchun130062, China
- Ren Hong-Lin
- Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, College of Animal Sciences, Jilin University, Xi An Da Lu 5333, Changchun130062, China
- DOI
- https://doi.org/10.2478/jvetres-2020-0033
- Journal volume & issue
-
Vol. 64,
no. 2
pp. 253 – 261
Abstract
The plate counting method widely used at present to discern viable from non-viable Brucella in the host or cell is time-consuming and laborious. Therefore, it is necessary to establish a rapid, simple method for detecting and counting viable Brucella organisms.
Keywords
