OZITX, a pertussis toxin-like protein for occluding inhibitory G protein signalling including Gαz

Alastair C. Keen; Maria Hauge Pedersen; Laura Lemel; Daniel J. Scott; Meritxell Canals; Dene R. Littler; Travis Beddoe; Yuki Ono; Lei Shi; Asuka Inoue; Jonathan A. Javitch; J. Robert Lane

doi:10.1038/s42003-022-03191-5

Communications Biology (Mar 2022)

OZITX, a pertussis toxin-like protein for occluding inhibitory G protein signalling including Gαz

Alastair C. Keen,
Maria Hauge Pedersen,
Laura Lemel,
Daniel J. Scott,
Meritxell Canals,
Dene R. Littler,
Travis Beddoe,
Yuki Ono,
Lei Shi,
Asuka Inoue,
Jonathan A. Javitch,
J. Robert Lane

Affiliations

Alastair C. Keen: Drug Discovery Biology, Monash Institute of Pharmaceutical Sciences, Monash University
Maria Hauge Pedersen: Departments of Psychiatry and Molecular Pharmacology and Therapeutics, Vagelos College of Physicians and Surgeons, Columbia University
Laura Lemel: Division of Physiology, Pharmacology and Neuroscience, School of Life Sciences, Queen’s Medical Centre, University of Nottingham
Daniel J. Scott: Department of Biochemistry and Molecular Biology, University of Melbourne
Meritxell Canals: Division of Physiology, Pharmacology and Neuroscience, School of Life Sciences, Queen’s Medical Centre, University of Nottingham
Dene R. Littler: Infection and Immunity Program and Department of Biochemistry and Molecular Biology, Biomedicine Discovery Institute, Monash University
Travis Beddoe: Department of Animal, Plant and Soil Science and Centre for AgriBioscience, La Trobe University
Yuki Ono: Graduate School of Pharmaceutical Sciences, Tohoku University
Lei Shi: Computational Chemistry and Molecular Biophysics Section, National Institute on Drug Abuse - Intramural Research Program, National Institutes of Health
Asuka Inoue: Graduate School of Pharmaceutical Sciences, Tohoku University
Jonathan A. Javitch: Departments of Psychiatry and Molecular Pharmacology and Therapeutics, Vagelos College of Physicians and Surgeons, Columbia University
J. Robert Lane: Division of Physiology, Pharmacology and Neuroscience, School of Life Sciences, Queen’s Medical Centre, University of Nottingham

DOI: https://doi.org/10.1038/s42003-022-03191-5
Journal volume & issue: Vol. 5, no. 1
pp. 1 – 10

Abstract

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A recently identified pertussis toxin-like AB5 toxin, OZITX, is found to inhibit Gαi/o and Gαz G proteins. In combination with directed mutations, it is a useful tool for interrogating Gαi/o/z G protein subunits individually.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

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