mBio
(Oct 2021)
Morphological and Transcriptional Responses to CRISPRi Knockdown of Essential Genes in Escherichia coli
Melanie R. Silvis,
Manohary Rajendram,
Handuo Shi,
Hendrik Osadnik,
Andrew N. Gray,
Spencer Cesar,
Jason M. Peters,
Cameron C. Hearne,
Parth Kumar,
Horia Todor,
Kerwyn Casey Huang,
Carol A. Gross
Affiliations
Melanie R. Silvis
Department of Cell and Tissue Biology, University of California San Francisco, San Francisco, California, USA
Manohary Rajendram
Department of Bioengineering, Stanford University, Stanford, California, USA
Handuo Shi
ORCiD Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California, USA
Hendrik Osadnik
Department of Cell and Tissue Biology, University of California San Francisco, San Francisco, California, USA
Andrew N. Gray
Department of Cell and Tissue Biology, University of California San Francisco, San Francisco, California, USA
Spencer Cesar
Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California, USA
Jason M. Peters
ORCiD Pharmaceutical Sciences Division, School of Pharmacy, University of Wisconsin—Madison, Madison, Wisconsin, USA
Cameron C. Hearne
Department of Cell and Tissue Biology, University of California San Francisco, San Francisco, California, USA
Parth Kumar
Department of Cell and Tissue Biology, University of California San Francisco, San Francisco, California, USA
Horia Todor
Department of Cell and Tissue Biology, University of California San Francisco, San Francisco, California, USA
Kerwyn Casey Huang
ORCiD Department of Bioengineering, Stanford University, Stanford, California, USA
Carol A. Gross
Department of Cell and Tissue Biology, University of California San Francisco, San Francisco, California, USA
DOI
https://doi.org/10.1128/mBio.02561-21
Journal volume & issue
Vol. 12,
no. 5
Abstract
Read online
Essential genes make up only ∼5 to 10% of the genetic complement in most organisms but occupy much of their protein synthesis and account for almost all antibiotic targets. Despite the importance of essential genes, their intractability has, until recently, hampered efforts to study them.
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