Grape polyphenols concentrate demonstrates cardioprotection in terms of hypoxic myocardial injury

Abstract

Objective — Beneficial effects of natural grape polyphenols on human health have been known for a long time and still attract more and more interest of many researchers. The aim of research was to reveal cardioprotective properties of polyphenols contained in grape concentrate "Fenocor" in rats exposed experimental histotoxic hypoxia. Material and Methods — The study was conducted on 21 adult male Wistar rats divided into 3 groups, 2 of which were administered CoCl2 water solution in 60 mg/kg dose for 7 days. The control group consisted of 5 non-exposed animals. The rats of the second group (n=8) after the administration of cobalt chloride did not receive any treatment, the animals of the third group (n=8) after administration of cobalt chloride were additionally given Fenocor – a grape polyphelols water solution at a dose of 2.5 ml/kg diluted in 0.05 ml of water. Morphological study was performed using light and electron microscopy. During the experiment the following biochemical parameters such as contents of malondialdehyde, oxidative modification of proteins were estimated. Results — The outcome of the cobalt toxic exposure on the heart of animals in experiments resulted in the development of severe cardiomyopathy which lead to the need for timely cardioprotection. Histological structure of myocardium observed in the second group of male rats after cobalt intoxication on the background of grape polyphenol concentrate generally reflected a tendency to minimize of the damage extent which was manifested in the form of normalization of cell structures and muscle fibers. Conclusion — Administration of Fenocor has demonstrated its antioxidant and cytoprotective properties, promoted myocardial structure preservation in rats exposed to histotoxic hypoxia.

Keywords

• cobalt
• rats
• grape polyphenols
• myocardium
• hypoxia
• experiment