Rapid Nanopore Whole-Genome Sequencing for Anthrax Emergency Preparedness

Abstract

Human anthrax cases necessitate rapid response. We completed Bacillus anthracis nanopore whole-genome sequencing in our high-containment laboratory from a human anthrax isolate hours after receipt. The de novo assembled genome showed no evidence of known antimicrobial resistance genes or introduced plasmid(s). Same-day genomic characterization enhances public health emergency response.

Keywords

• anthrax
• Bacillus anthracis
• bacteria
• whole-genome sequencing
• nanopore
• emergency preparedness