Nature Communications (Nov 2019)

Cytoplasmic glycoengineering enables biosynthesis of nanoscale glycoprotein assemblies

  • Hanne L. P. Tytgat,
  • Chia-wei Lin,
  • Mikail D. Levasseur,
  • Markus B. Tomek,
  • Christoph Rutschmann,
  • Jacqueline Mock,
  • Nora Liebscher,
  • Naohiro Terasaka,
  • Yusuke Azuma,
  • Michael Wetter,
  • Martin F. Bachmann,
  • Donald Hilvert,
  • Markus Aebi,
  • Timothy G. Keys

DOI
https://doi.org/10.1038/s41467-019-13283-2
Journal volume & issue
Vol. 10, no. 1
pp. 1 – 10

Abstract

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Established bacterial glycoengineering platforms limit access to protein and glycan substrates. Here the authors design a cytoplasmic protein glycosylation system, Glycoli, to generate a variety of multivalent glycostructures.