Cloning and identification of PK15 cells for enhanced replication of classical swine fever virus

Abstract

Classical swine fever virus (CSFV) causes an economically important and highly contagious disease of pigs, leading to economic losses around the world. Attenuated live vaccines with CSFV antigens have played an important role in the prevention and control of the disease. Porcine kidney 15 (PK15) cells have been widely used for the propagation of CSFV, but this cell line is not efficient or homogeneously susceptible to viral infection.

Keywords

• classical swine fever virus
• pk15
• high-permissive cells
• cell cloning
• vaccine production