Animals (Aug 2023)

Residue Depletion Profile and Estimation of Withdrawal Period for Sulfadimethoxine and Ormetoprim in Edible Tissues of Nile Tilapia (<i>Oreochromis</i> sp.) on Medicated Feed

  • Lucas Victor Pereira de Freitas,
  • Carlos Augusto Alvarenga da Mota Júnior,
  • Marina Alves Damaceno,
  • Juliana Grell Fernandes Silveira,
  • Ana Carolina Vellosa Portela,
  • Sarah Chagas Campanharo,
  • Agnaldo Fernando Baldo da Silva,
  • Inácio Mateus Assane,
  • Fabiana Pilarski,
  • James Jacob Sasanya,
  • Jonas Augusto Rizzato Paschoal

DOI
https://doi.org/10.3390/ani13152499
Journal volume & issue
Vol. 13, no. 15
p. 2499

Abstract

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Sulfadimethoxine (SDM) and ormetoprim (OMP) are antimicrobials used in combination to treat bacterial infections in fish farming. The use of this drug combination is not yet regulated in some countries, such as Brazil. Due to the lack of regulated drugs for aquaculture in Brazil, this study investigated the residue depletion profile of SDM and OMP in Nile tilapia (Oreochromis sp.) after oral administration. Fish were treated with medicated feed containing a 5:1 ratio of SDM:OMP at the dose of 50 mg kg BW−1 for five consecutive days with an average water temperature of 28 °C. The drugs were incorporated into the feed by using a gelatin coating process which promoted homogeneity in drug concentration and prevented the drug leaching into the water during medication. The SDM and OMP determination in fish fillets (muscle plus skin in natural proportions) was performed using the QuEChERS approach followed by LC-MS/MS quantification. The analytical method was validated according to Brazilian and selected international guidelines. A withdrawal period of 9 days (or 252 °C days) was estimated for the sum of SDM and OMP residues at concentration levels below the maximum residue level of 100 µg kg−1.

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