PLoS ONE (Jan 2016)

Development of an Indirect ELISA for Serological Diagnosis of Bovine herpesvirus 5.

  • Luana A Dummer,
  • Itauá L Araujo,
  • Fabrício S Campos,
  • Matheus C da Rosa,
  • Paula F Finger,
  • Patricia D de Oliveira,
  • Fabricio R Conceição,
  • Geferson Fischer,
  • Paulo M Roehe,
  • Fábio P L Leite

DOI
https://doi.org/10.1371/journal.pone.0149134
Journal volume & issue
Vol. 11, no. 2
p. e0149134

Abstract

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Bovine herpesviruses 1 and 5 (BoHV-1 and BoHV-5) are economically important pathogens, associated with a variety of clinical syndromes, including respiratory and genital disease, reproductive failure and meningoencephalitis. The standard serological assay to diagnose BoHV-1 and BoHV-5 infections is the virus neutralization test (VNT), a time consuming procedure that requires manipulation of infectious virus. In the present study a highly sensitive and specific single dilution indirect ELISA was developed using recombinant glycoprotein D from BoHV-5 as antigen (rgD5ELISA). Bovine serum samples (n = 450) were screened by VNT against BoHV-5a and by rgD5ELISA. Compared with the VNT, the rgD5ELISA demonstrated accuracy of 99.8%, with 100% sensitivity, 96.7% specificity and coefficient of agreement between the tests of 0.954. The rgD5ELISA described here shows excellent agreement with the VNT and is shown to be a simple, convenient, specific and highly sensitive virus-free assay for detection of serum antibodies to BoHV-5.