Primer Extension Analysis of HBV DNA with Strand-Specific Primers
Jaesung Jung,
Kyongmin Kim
Affiliations
Jaesung Jung
Department of Microbiology, Ajou University School of Medicine, Suwon, KoreaDepartment of Biomedical Science, Graduate School of Ajou University, Suwon, Korea
Kyongmin Kim
Department of Microbiology, Ajou University School of Medicine, Suwon, KoreaDepartment of Microbiology, Ajou University School of Medicine, Suwon, Korea, Department of Biomedical Science, Ajou University School of Medicine, Suwon, Korea
We performed primer extension assay to determine which steps of HBV DNA synthesis (i.e., minus- and plus-strand DNA synthesis and circularization of RC DNA) are affected by phosphoacceptor site mutations in C protein. In these experiments, we used several specific oligonucleotide primers. For quantitation, the level of extended DNA (ED) was normalized to the level of a single internal standard (IS) DNA.
