Testing authenticity of certified paddy seed with user friendly morpho-molecular tool- kit

Abstract

In the present investigation, twenty nine rice cultivars were characterized using grain morphological characters and molecular markers to develop a morpho- molecular tool kit for application in authenticity testing of the certified seed lot and in maintenance breeding. The study suggested that identification of rice varieties is possible with grain morphology and molecular markers. Using grain morphological characters, seven varieties were uniquely identified viz., Prafulla, Numali, Dikhow, Dhanashree, Basundhara, Jalkuwari and Bhugali. Molecular fingerprints were generated based on SSR polymorphism. Using the present set of SSR markers, eight rice varieties viz., Gitesh, Jalashree, Ranjit, Jaymati, Bhugali, Ranjit sub-1, Numali, and Piyali, were distinguished with RM 12, RM 25, RM 190, RM 307 and RM 287on the basis of the unique banding pattern. In the phylogenetic relationship based on grain morphology, Dikhow was identified as an outlier, which is unique for the grain length, whereas based on SSR data, variety Praffulla was identified as an outlier with unique medium grain width.

Keywords

• rice
• ssr marker
• diagnostic grain characters
• genetic purity