Draft genome sequence of a clinical Acinetobacter haemolyticus isolate from South Africa

Abstract

Objectives: Here we describe the draft genome sequence of a clinical Acinetobacter haemolyticus isolate (A109R1B4) from a rectal swab of a hospitalised patient in South Africa. Methods: Genomic DNA from the isolate was sequenced using an Illumina MiSeq platform. Generated reads were de novo assembled using Qiagen CLC Genomics Workbench. The assembled contigs were annotated and antimicrobial resistance genes and sequence type were identified. Results: The genome comprised 281 contigs with a total assembly length of 3 371 389 bp, a G + C content of 39.9% and an N50 value of 58 196 bp, and reference coverage was 93.08 while the coverage was 88.08. A total of 3387 genes, 3292 coding sequences (CDS), 3175 coding genes and 95 RNA genes were detected. The antimicrobial resistance genes blaOXA-264 and aac(6ʹ)-Ig were detected. Conclusion: The genome sequence reported will serve as a reference point for molecular epidemiological studies of antibiotic-resistant A. haemolyticus in Africa.

Keywords

• Whole-genome sequencing
• Antimicrobial resistance
• Acinetobacter haemolyticus
• Carriage
• South Africa