Effects of Probiotic Supplementation on Selected Parameters of Blood Prooxidant‐Antioxidant Balance in Elite Athletes: A Double‐Blind Randomized Placebo‐Controlled Study

Journal of Human Kinetics. 2018;64(1):111-122 DOI 10.1515/hukin-2017-0203

 

Journal Homepage

Journal Title: Journal of Human Kinetics

ISSN: 1899-7562 (Online)

Publisher: Sciendo

Society/Institution: The Jerzy Kukuczka Academy of Physical Education in Katowice

LCC Subject Category: Medicine: Internal medicine: Special situations and conditions: Sports medicine

Country of publisher: Poland

Language of fulltext: English

Full-text formats available: PDF

 

AUTHORS


Michalickova Danica (Institute of Pharmacology, First Faculty of Medicine, Charles University, Prague, Czech Republic)

Kotur-Stevuljevic Jelena (Department of Medical Biochemistry, Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia)

Miljkovic Milica (Department of Medical Biochemistry, Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia)

Dikic Nenad (Sports Medicine Association of Serbia, Belgrade, Serbia)

Kostic-Vucicevic Marija (Sports Medicine Association of Serbia, Belgrade, Serbia)

Andjelkovic Marija (Sports Medicine Association of Serbia, Belgrade, Serbia)

Koricanac Vladimir (Institute of Meat Hygiene and Technology, Belgrade, Serbia)

Djordjevic Brizita (Department of Bromatology, Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia)

EDITORIAL INFORMATION

Peer review

Editorial Board

Instructions for authors

Time From Submission to Publication: 7 weeks

 

Abstract | Full Text

A randomized, double-blind, placebo-controlled study was conducted, in order to evaluate if Lactobacillus helveticus Lafti® L10 (Lallemand Health Solutions, Montreal, Canada) supplementation during three months could influence oxidative markers in the population of elite athletes: triathletes, cyclists and endurance athletes. Twenty-two elite athletes were randomized to either placebo (n = 12) or probiotic (n = 10) groups. The probiotic group received 2x1010 colony forming units of Lafti® L10. Before and after the supplementation serum samples were collected. Markers of oxidative stress and anti-oxidative defense: superoxide dismutase (SOD), paraoxonase (PON), advanced oxidation protein products (AOPP), malondialdehyde (MDA), total antioxidant status, total oxidant status, pro-oxidant-antioxidant balance, oxidative stress index, bilirubin, uric acid and albumin were determined in serum. Parameters of lipid status, as well as susceptibility to copper-induced oxidation of LDL particles in vitro were also determined. There was a significant interaction effect for MDA (p = 0.039), with a decrease in MDA in the probiotic group only (p = 0.049). There was a significant interaction effect for AOPP (p = 0.037), with a significant decrease in the probiotic group (p = 0.045). Interaction effect for SOD was approaching to formal significance (p = 0.108) and the post-hoc test showed a significant decrease in the probiotic group (p = 0.041) only. A significant correlation between AOPP and SOD (p = 0.012, r = -0.40) was found in the probiotic group at the end of the study. PON1 activity was decreased in both the probiotic (p = 0.032) and placebo group (p = 0.035). No significant changes in the remainder of the evaluated parameters were noted. In conclusion, probiotic strain Lafti® L10 exerts certain antioxidant potential, but further research is needed.