Identification of surfactin lipopeptides isoforms produced by a newly isolated strain Bacillus sp. MI27 with potential use as main ingredients in detergent formulation: Application of mixture design for the formulation of a bio-based detergent

Abstract

The purification and characterization of lipopeptide biosurfactants produced by Bacillus sp. MI27 permits to identify two clusters of cyclic and linear surfactin isoforms. Aiming for a potential application in industrial biotechnology, we studied its potential washing capacity. The results show a good ability to wash oil stains, motor oil and mayonnaise evaluated by the determination of the % of stain removal and tissue bleaching indices after washing. Optimal washing capacities were unregistered for 300 and 400 mg/L at pH values ranging from 5 to 9 and temperatures ranging from 20 to 40°C. Using a 4-factor mixing design, we optimized a liquid formula composed only of BioS; silicate; carboxymethyl cellulose and calcium phosphate. The washing efficiency of the formula is of the order of 43.46% for petroleum and 49.10% for motor oil with a percentage reduction in surface tension of about 55.79% corresponding to 25.7 mN/m. HIGHLIGHTS Identification of cyclic and linear surfactin isoforms from Bacillus sp. MI27.; Optimal washing capacities for 300–400 mg/L at pH values 5–9 and temperatures 20–40 °C.; Mixture design for optimized liquid formula composed only of BioS, silicate, carboxymethyl cellulose and calcium phosphate.; The washing efficiency of the formula is 43.46% for petroleum and 49.10% for motor oil.; Surface tension value of 25.7 mN/m.;

Keywords

• biosurfactant
• detergent
• formulation
• mixture design
• surfactin
• washing capacity