Synergy between Indoloquinolines and Ciprofloxacin: An Antibiofilm Strategy against <i>Pseudomonas aeruginosa</i>
Emilie Charpentier,
Ludovic Doudet,
Ingrid Allart-Simon,
Marius Colin,
Sophie C. Gangloff,
Stéphane Gérard,
Fany Reffuveille
Affiliations
Emilie Charpentier
EA 4691 Biomatériaux et Inflammation en Site Osseux (BIOS), UFR Pharmacie, Université de Reims Champagne-Ardenne, SFR Cap Santé (FED 4231), 51097 Reims, France
Ludovic Doudet
Institut de Chimie Moléculaire de Reims (ICMR-UMR CNRS 7312), UFR Pharmacie, Université de Reims Champagne-Ardenne, 51097 Reims, France
Ingrid Allart-Simon
Institut de Chimie Moléculaire de Reims (ICMR-UMR CNRS 7312), UFR Pharmacie, Université de Reims Champagne-Ardenne, 51097 Reims, France
Marius Colin
EA 4691 Biomatériaux et Inflammation en Site Osseux (BIOS), UFR Pharmacie, Université de Reims Champagne-Ardenne, SFR Cap Santé (FED 4231), 51097 Reims, France
Sophie C. Gangloff
EA 4691 Biomatériaux et Inflammation en Site Osseux (BIOS), UFR Pharmacie, Université de Reims Champagne-Ardenne, SFR Cap Santé (FED 4231), 51097 Reims, France
Stéphane Gérard
Institut de Chimie Moléculaire de Reims (ICMR-UMR CNRS 7312), UFR Pharmacie, Université de Reims Champagne-Ardenne, 51097 Reims, France
Fany Reffuveille
EA 4691 Biomatériaux et Inflammation en Site Osseux (BIOS), UFR Pharmacie, Université de Reims Champagne-Ardenne, SFR Cap Santé (FED 4231), 51097 Reims, France
Antibiotic treatments can participate in the formation of bacterial biofilm in case of under dosage. The interest of indoloquinoline scaffold for drug discovery incited us to study the preparation of new indolo [2,3-b]quinoline derivatives by a domino radical process. We tested the effect of two different “indoloquinoline” molecules (Indol-1 and Indol-2) without antimicrobial activity, in addition to ciprofloxacin, on biofilm formation thanks to crystal violet staining and enumeration of adhered bacteria. This association of ciprofloxacin and Indol-1 or Indol-2 attenuated the formation of biofilm up to almost 80% compared to ciprofloxacin alone, or even prevented the presence of adhered bacteria. In conclusion, these data prove that the association of non-antimicrobial molecules with an antibiotic can be a solution to fight against biofilm and antibiotic resistance emergence.
