Generation of an induced pluripotent stem cell line (CSBZZUi001-A) from a female Alzheimer’s patient carrying the PSEN1 709 T > C heterozygous mutation

Xiao-Yan Meng; Hui-Fang Sun; Ying-Jie Zhang; Wen-Xiu Li; Dian-Dian Wang; Dan-Hua Liu; Sen-Miao Li; Yue Wu; Jia-Kang Li; Xiao-Hui Liu; Pei-Pei Liu; Run-Zhou Yang; Shu-Ang Li; Jian-Sheng Kang

Stem Cell Research (Sep 2024)

Generation of an induced pluripotent stem cell line (CSBZZUi001-A) from a female Alzheimer’s patient carrying the PSEN1 709 T > C heterozygous mutation

Xiao-Yan Meng,
Hui-Fang Sun,
Ying-Jie Zhang,
Wen-Xiu Li,
Dian-Dian Wang,
Dan-Hua Liu,
Sen-Miao Li,
Yue Wu,
Jia-Kang Li,
Xiao-Hui Liu,
Pei-Pei Liu,
Run-Zhou Yang,
Shu-Ang Li,
Jian-Sheng Kang

Affiliations

Xiao-Yan Meng: Clinical Systems Biology Laboratories, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China; The Academy of Medical Sciences, Zhengzhou University, Zhengzhou 450001, China; Department of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou University, 450000 Zhengzhou, Henan, China
Hui-Fang Sun: Clinical Systems Biology Laboratories, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China; Department of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou University, 450000 Zhengzhou, Henan, China
Ying-Jie Zhang: Clinical Systems Biology Laboratories, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China; The Academy of Medical Sciences, Zhengzhou University, Zhengzhou 450001, China; Department of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou University, 450000 Zhengzhou, Henan, China
Wen-Xiu Li: Clinical Systems Biology Laboratories, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China; The Academy of Medical Sciences, Zhengzhou University, Zhengzhou 450001, China; Department of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou University, 450000 Zhengzhou, Henan, China
Dian-Dian Wang: Clinical Systems Biology Laboratories, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China; The Academy of Medical Sciences, Zhengzhou University, Zhengzhou 450001, China; Department of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou University, 450000 Zhengzhou, Henan, China
Dan-Hua Liu: Clinical Systems Biology Laboratories, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China; The Academy of Medical Sciences, Zhengzhou University, Zhengzhou 450001, China; Department of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou University, 450000 Zhengzhou, Henan, China
Sen-Miao Li: Clinical Systems Biology Laboratories, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China; The Academy of Medical Sciences, Zhengzhou University, Zhengzhou 450001, China; Department of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou University, 450000 Zhengzhou, Henan, China
Yue Wu: Clinical Systems Biology Laboratories, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China; The Academy of Medical Sciences, Zhengzhou University, Zhengzhou 450001, China; Department of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou University, 450000 Zhengzhou, Henan, China
Jia-Kang Li: Clinical Systems Biology Laboratories, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China; The Academy of Medical Sciences, Zhengzhou University, Zhengzhou 450001, China; Department of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou University, 450000 Zhengzhou, Henan, China
Xiao-Hui Liu: Clinical Systems Biology Laboratories, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China; The Academy of Medical Sciences, Zhengzhou University, Zhengzhou 450001, China; Department of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou University, 450000 Zhengzhou, Henan, China
Pei-Pei Liu: Clinical Systems Biology Laboratories, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China
Run-Zhou Yang: Clinical Systems Biology Laboratories, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China
Shu-Ang Li: Clinical Systems Biology Laboratories, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China; Corresponding authors.
Jian-Sheng Kang: Clinical Systems Biology Laboratories, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China; Corresponding authors.

Journal volume & issue: Vol. 79
p. 103486

Abstract

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Pluripotent stem cells were generated through the electroporation of episomal plasmids, containing crucial reprogramming factors, into skin fibroblasts extracted from a female Alzheimer’s patient harboring the PSEN1 709 T > C (p.Phe237Leu) heterozygous mutation. The pluripotent stem cells exhibit a normal karyotype and express pivotal stem cell markers including TRA-1-60, Nanog, SOX2, and OCT4. Furthermore, their capacity to differentiate into the three germ layers in in vivo teratoma experiments has been substantiated. The pluripotent stem cell line can serve as a cellular model for Alzheimer’s disease, offering significant value in elucidating the pathogenesis and therapeutic strategies of the disease.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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