Development of a prognostic prediction model based on a combined multi-omics analysis of head and neck squamous cell carcinoma cell pyroptosis-related genes

Bin Chen; Bin Chen; Bin Chen; Yuanbo Luo; Yuanbo Luo; Yuanbo Luo; Xueran Kang; Xueran Kang; Xueran Kang; Yuxing Sun; Yuxing Sun; Yuxing Sun; Chenyan Jiang; Chenyan Jiang; Chenyan Jiang; Bin Yi; Bin Yi; Bin Yi; Xiaojun Yan; Xiaojun Yan; Xiaojun Yan; Yisheng Chen; Runjie Shi; Runjie Shi; Runjie Shi

doi:10.3389/fgene.2022.981222

Frontiers in Genetics (Sep 2022)

Development of a prognostic prediction model based on a combined multi-omics analysis of head and neck squamous cell carcinoma cell pyroptosis-related genes

Bin Chen,
Bin Chen,
Bin Chen,
Yuanbo Luo,
Yuanbo Luo,
Yuanbo Luo,
Xueran Kang,
Xueran Kang,
Xueran Kang,
Yuxing Sun,
Yuxing Sun,
Yuxing Sun,
Chenyan Jiang,
Chenyan Jiang,
Chenyan Jiang,
Bin Yi,
Bin Yi,
Bin Yi,
Xiaojun Yan,
Xiaojun Yan,
Xiaojun Yan,
Yisheng Chen,
Runjie Shi,
Runjie Shi,
Runjie Shi

Affiliations

Bin Chen: Department of Otorhinolaryngology Head and Neck Surgery, Ninth People’s Hospital, Shanghai Jiaotong University, School of Medicine, Shanghai, China
Bin Chen: Ear Institute, School of Medicine, Shanghai Jiaotong University, Shanghai, China
Bin Chen: Shanghai Key Laboratory of Translational Medicine on Ear and Nose Diseases, Shanghai, China
Yuanbo Luo: Department of Otorhinolaryngology Head and Neck Surgery, Ninth People’s Hospital, Shanghai Jiaotong University, School of Medicine, Shanghai, China
Yuanbo Luo: Ear Institute, School of Medicine, Shanghai Jiaotong University, Shanghai, China
Yuanbo Luo: Shanghai Key Laboratory of Translational Medicine on Ear and Nose Diseases, Shanghai, China
Xueran Kang: Department of Otorhinolaryngology Head and Neck Surgery, Ninth People’s Hospital, Shanghai Jiaotong University, School of Medicine, Shanghai, China
Xueran Kang: Ear Institute, School of Medicine, Shanghai Jiaotong University, Shanghai, China
Xueran Kang: Shanghai Key Laboratory of Translational Medicine on Ear and Nose Diseases, Shanghai, China
Yuxing Sun: Department of Otorhinolaryngology Head and Neck Surgery, Ninth People’s Hospital, Shanghai Jiaotong University, School of Medicine, Shanghai, China
Yuxing Sun: Ear Institute, School of Medicine, Shanghai Jiaotong University, Shanghai, China
Yuxing Sun: Shanghai Key Laboratory of Translational Medicine on Ear and Nose Diseases, Shanghai, China
Chenyan Jiang: Department of Otorhinolaryngology Head and Neck Surgery, Ninth People’s Hospital, Shanghai Jiaotong University, School of Medicine, Shanghai, China
Chenyan Jiang: Ear Institute, School of Medicine, Shanghai Jiaotong University, Shanghai, China
Chenyan Jiang: Shanghai Key Laboratory of Translational Medicine on Ear and Nose Diseases, Shanghai, China
Bin Yi: Department of Otorhinolaryngology Head and Neck Surgery, Ninth People’s Hospital, Shanghai Jiaotong University, School of Medicine, Shanghai, China
Bin Yi: Ear Institute, School of Medicine, Shanghai Jiaotong University, Shanghai, China
Bin Yi: Shanghai Key Laboratory of Translational Medicine on Ear and Nose Diseases, Shanghai, China
Xiaojun Yan: Department of Otorhinolaryngology Head and Neck Surgery, Ninth People’s Hospital, Shanghai Jiaotong University, School of Medicine, Shanghai, China
Xiaojun Yan: Ear Institute, School of Medicine, Shanghai Jiaotong University, Shanghai, China
Xiaojun Yan: Shanghai Key Laboratory of Translational Medicine on Ear and Nose Diseases, Shanghai, China
Yisheng Chen: Department of Sports Medicine, Huashan Hospital, Fudan University, Shanghai, China
Runjie Shi: Department of Otorhinolaryngology Head and Neck Surgery, Ninth People’s Hospital, Shanghai Jiaotong University, School of Medicine, Shanghai, China
Runjie Shi: Ear Institute, School of Medicine, Shanghai Jiaotong University, Shanghai, China
Runjie Shi: Shanghai Key Laboratory of Translational Medicine on Ear and Nose Diseases, Shanghai, China

DOI: https://doi.org/10.3389/fgene.2022.981222
Journal volume & issue: Vol. 13

Abstract

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This study aimed to understand the prognosis of patients with head and neck squamous cell carcinoma (HNSCC) and to develop and validate a prognostic model for HNSCC based on pyroptosis-associated genes (PAGs) in nasopharyngeal carcinoma. The Cancer Genome Atlas database was used to identify differentially expressed PAGs. These genes were analyzed using the Kyoto Encyclopedia of Genes and Genomes functional annotation analyses and Gene Ontology analyses. The NLR family pyrin domain containing 1 (NLRP1) gene, charged multivesicular body protein 7 (CHMP7) gene, and cytochrome C (CYCS) gene were used to create a prognostic model for HNSCC. The results of the Kaplan-Meier (K-M) and Cox regression analyses indicated that the developed model served as an independent risk factor for HNSCC. According to the K-M analysis, the overall survival of high-risk patients was lower than that of low-risk patients. The hazard ratios corresponding to the risk scores determined using the multivariate and univariate Cox regression analyses were 1.646 (95% confidence interval (CI): 1.189–2.278) and 1.724 (95% CI: 1.294–2.298), respectively, and the area under the receiver operator characteristic curve was 0.621. The potential mechanisms associated with the functions of the identified genes were then identified, and the tumor microenvironment and levels of immune cell infiltration achieved were analyzed. The immune infiltration analysis revealed differences in the distribution of Th cells, tumor-infiltrating lymphocytes, regulatory T cells, follicular helper T cells, adipose-derived cells, interdigitating dendritic cells, CD8+ T cells, and B cells. However, validating bioinformatics analyses through biological experiments is still recommended. This study developed a prognostic model for HNSCC that included NLRP1, CHMP7, and CYCS.

Published in Frontiers in Genetics

ISSN: 1664-8021 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Science: Biology (General): Genetics
Website: http://journal.frontiersin.org/journal/genetics

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