Ambrosia fungi are well-known for their symbiotic interactions with ambrosia beetles, acting as a sole food source of larvae and adult beetles. As a first step to reveal these interactions, extensive survey on the fungal symbionts of ambrosia beetles dwelling in Korea. Eight fungal species isolated from 15 ambrosia beetle species were not known for their presence in Korea. Seven of these belonged to two orders of Ascomycota; Microascales (Ambrosiella beaveri, A. catenulate, and A. roeperi) and Ophiostomatales (Leptographium verrucosum, Raffaelea cyclorhipidii, R. subfusca, and Sporothrix eucastaneae) and one to Polyporales of Basidiomycota (Irpex subulatus). This is the first report of these species in Korea with taxonomic descriptions.