Communications Biology (Mar 2021)

smiFISH and embryo segmentation for single-cell multi-gene RNA quantification in arthropods

  • Llilians Calvo,
  • Matthew Ronshaugen,
  • Tom Pettini

DOI
https://doi.org/10.1038/s42003-021-01803-0
Journal volume & issue
Vol. 4, no. 1
pp. 1 – 12

Abstract

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Here, the authors combine single-molecule inexpensive FISH (smiFISH) with cell membrane immunofluorescence and mathematical methods to enable whole embryo segmentation in 3D image stacks and mRNA quantification of multiple genes in each cell of the embryo.