Molecular Strategies for Intensity-Dependent Olfactory Processing in Caenorhabditis elegans

Hankui Cheng; Hankui Cheng; Hankui Cheng; Yu Liu; Yu Liu; Yu Liu; Yadan Xue; Yadan Xue; Jiajie Shao; Jiajie Shao; Zhibing Tan; Zhibing Tan; Siyan Liu; Siyan Liu; Siyan Liu; Shumin Duan; Shumin Duan; Shumin Duan; Shumin Duan; Lijun Kang; Lijun Kang; Lijun Kang

doi:10.3389/fnmol.2021.748214

Frontiers in Molecular Neuroscience (Nov 2021)

Molecular Strategies for Intensity-Dependent Olfactory Processing in Caenorhabditis elegans

Hankui Cheng,
Hankui Cheng,
Hankui Cheng,
Yu Liu,
Yu Liu,
Yu Liu,
Yadan Xue,
Yadan Xue,
Jiajie Shao,
Jiajie Shao,
Zhibing Tan,
Zhibing Tan,
Siyan Liu,
Siyan Liu,
Siyan Liu,
Shumin Duan,
Shumin Duan,
Shumin Duan,
Shumin Duan,
Lijun Kang,
Lijun Kang,
Lijun Kang

Affiliations

Hankui Cheng: Department of Neurobiology, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China
Hankui Cheng: Department of Neurosurgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China
Hankui Cheng: NHC and CAMS Key Laboratory of Medical Neurobiology, MOE Frontier Science Center for Brain Research and Brain-Machine Integration, School of Brain Science and Brain Medicine, Zhejiang University, Hangzhou, China
Yu Liu: Department of Neurobiology, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China
Yu Liu: Department of Neurosurgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China
Yu Liu: NHC and CAMS Key Laboratory of Medical Neurobiology, MOE Frontier Science Center for Brain Research and Brain-Machine Integration, School of Brain Science and Brain Medicine, Zhejiang University, Hangzhou, China
Yadan Xue: Department of Neurobiology, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China
Yadan Xue: Department of Neurosurgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China
Jiajie Shao: Department of Neurobiology, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China
Jiajie Shao: Department of Neurosurgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China
Zhibing Tan: Department of Neurobiology, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China
Zhibing Tan: Department of Neurosurgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China
Siyan Liu: Department of Neurobiology, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China
Siyan Liu: Department of Neurosurgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China
Siyan Liu: NHC and CAMS Key Laboratory of Medical Neurobiology, MOE Frontier Science Center for Brain Research and Brain-Machine Integration, School of Brain Science and Brain Medicine, Zhejiang University, Hangzhou, China
Shumin Duan: Department of Neurobiology, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China
Shumin Duan: Department of Neurosurgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China
Shumin Duan: NHC and CAMS Key Laboratory of Medical Neurobiology, MOE Frontier Science Center for Brain Research and Brain-Machine Integration, School of Brain Science and Brain Medicine, Zhejiang University, Hangzhou, China
Shumin Duan: Department of Neurology, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China
Lijun Kang: Department of Neurobiology, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China
Lijun Kang: Department of Neurosurgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China
Lijun Kang: NHC and CAMS Key Laboratory of Medical Neurobiology, MOE Frontier Science Center for Brain Research and Brain-Machine Integration, School of Brain Science and Brain Medicine, Zhejiang University, Hangzhou, China

DOI: https://doi.org/10.3389/fnmol.2021.748214
Journal volume & issue: Vol. 14

Abstract

Read online

Various odorants trigger complex animal behaviors across species in both quality- and quantity-dependent manners. However, how the intensity of olfactory input is encoded remains largely unknown. Here we report that isoamyl alcohol (IAA) induces bi-directional currents through a Gα- guanylate cyclase (GC)- cGMP signaling pathway in Caenorhabditis elegans olfactory neuron amphid wing “C” cell (AWC), while two opposite cGMP signaling pathways are responsible for odor-sensing in olfactory neuron amphid wing “B” cell (AWB): (1) a depolarizing Gα (GPA-3)- phosphodiesterase (PDE) – cGMP pathway which can be activated by low concentrations of isoamyl alcohol (IAA), and (2) a hyperpolarizing Gα (ODR-3)- GC- cGMP pathway sensing high concentrations of IAA. Besides, IAA induces Gα (ODR-3)-TRPV(OSM-9)-dependent currents in amphid wing “A” cell (AWA) and amphid neuron “H” cell with single ciliated sensory ending (ASH) neurons with different thresholds. Our results demonstrate that an elaborate combination of multiple signaling machineries encode the intensity of olfactory input, shedding light on understanding the molecular strategies on sensory transduction.

Published in Frontiers in Molecular Neuroscience

ISSN: 1662-5099 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Medicine: Internal medicine: Neurosciences. Biological psychiatry. Neuropsychiatry
Website: https://www.frontiersin.org/journals/molecular-neuroscience

About the journal

Abstract

Keywords